NDLI: Low efficiency of functional translation of ouabain-resistant 2-Na(+),K(+)-ATPase mRNA in C7-MDCK epithelial cells.

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Low efficiency of functional translation of ouabain-resistant 2-Na(+),K(+)-ATPase mRNA in C7-MDCK epithelial cells.

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Low efficiency of functional translation of ouabain-resistant 2-Na(+),K(+)-ATPase mRNA in C7-MDCK epithelial cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Akimova, Olga A. Van Huysse, James Tremblay, Johanne Orlov, Sergei N.
Description	Country affiliation: Canada Author Affiliation: Akimova OA ( Research Centre, Centre hospitalier de l'Université de Montréal (CRCHUM) - Technopôle Angus, 2901 Rachel East, Montreal, QC H1W 4A4, Canada.)
Abstract	Na(+),K(+)-ATPase is a heterodimer consisting of catalytic 1- 4 and regulatory ß1-ß3 subunits. Recently, we reported that transfection with ouabain-resistant 1R-Na(+),K(+)-ATPase rescues renal epithelial C7-MDCK cells exclusively expressing the ouabain-sensitive 1S-isoform from the cytotoxic action of ouabain. To explore the role of 2 subunit in ion transport and cytotoxic action of ouabain, we compared the effect of ouabain on K(+) ((86)Rb) influx and the survival of ouabain-treated C7-MDCK cells stably transfected with 1R- and 2R-Na(+),K(+)-ATPase. 2R mRNA in transfected cells was â ¼8-fold more abundant than 1R mRNA, whereas immunoreactive 2R protein content was 5-fold lower than endogenous 1S protein. A concentration of 10 µmol/L ouabain led to complete inhibition of (86)Rb influx both in mock- and 2R-transfected cells, whereas maximal inhibition of (86)Rb influx in 1R-transfectd cells was observed at 1000 µmol/L ouabain. In contrast to the massive death of mock- and 2R-transfected cells exposed to 3 µmol/L ouabain , 1R-cells survived after 24 h incubation with 1000 µmol/L ouabain. Thus, our results show that unlike 1R, the presence of 2R-Na(+),K(+)-ATPase subunit mRNA and immunoreactive protein does not contribute to Na(+)/K(+) pump activity, and does not rescue C7-MDCK cells from the cytotoxic action of ouabain. Our results also suggest that the lack of impact of transfected 2-Na(+),K(+)-ATPase on Na(+)/K(+) pump activity and cell survival can be attributed to the low efficiency of its translation and (or) delivery to the plasma membrane of renal epithelial cells.
File Format	HTM / HTML
ISSN	00084212
e-ISSN	12057541
Journal	Canadian Journal of Physiology and Pharmacology
Issue Number	1
Volume Number	90
Language	English
Publisher	Canadian Science Publishing
Publisher Date	2012-01-01
Publisher Place	Canada
Access Restriction	Open
Subject Keyword	Discipline Physiology Discipline Pharmacology Epithelial Cells Metabolism Physiology Ouabain Adverse Effects Protein Biosynthesis Genetics Rna Isoforms Sodium-potassium-exchanging Atpase Biosynthesis Animals Cell Death Drug Effects Cells, Cultured Ion Transport Isoenzymes Kidney Potassium Rubidium Radioisotopes Transfection Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Pharmacology

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