NDLI: Chemical and biological characterization of sclerosin, an antifungal lipopeptide.

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Chemical and biological characterization of sclerosin, an antifungal lipopeptide.

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Chemical and biological characterization of sclerosin, an antifungal lipopeptide.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Berry, Chrystal L. Brassinga, Ann Karen C. Donald, Lynda J. Fernando, W. G. Dilantha Loewen, Peter C. de Kievit, Teresa R.
Description	Country affiliation: Canada Author Affiliation: Berry CL ( Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.)
Abstract	Pseudomonas sp. strain DF41 produces a lipopeptide, called sclerosin that inhibits the fungal pathogen Sclerotinia sclerotiorum . The aim of the current study was to deduce the chemical structure of this lipopeptide and further characterize its bioactivity. Mass spectrometry analysis determined the structure of sclerosin to be CH(3)-(CH(2))(6)-CH(OH)-CH(2)-CO-Dhb-Pro-Ala-Leu/Ile-Ala-Val-Val-Dhb-Thr-Val-Leu/Ile-Dhp-Ala-Ala-Ala-Val-Dhb-Dhb-Ala-Dab-Ser-Val-OH, similar to corpeptins A and B of the tolaasin group, differing by only 3 amino acids in the peptide chain. Subjecting sclerosin to various ring opening procedures revealed no new ions, suggesting that this molecule is linear. As such, sclerosin represents a new member of the tolaasin lipopeptide group. Incubation of S. sclerotinia ascospores and sclerotia in the presence of sclerosin inhibited the germination of both cell types. Sclerosin also exhibited antimicrobial activity against Bacillus species. Conversely, this lipopeptide demonstrated no zoosporicidal activity against the oomycete pathogen Phytophthora infestans . Next, we assessed the effect of DF41 and a lipopeptide-deficient mutant on the growth and development of Caenorhabditis elegans larvae. We discovered that sclerosin did not protect DF41 from ingestion by and degradation in the C. elegans digestive tract. However, another metabolite produced by this bacterium appeared to shorten the life-span of the nematode compared to C. elegans growing on Escherichia coli OP50.
File Format	HTM / HTML
ISSN	00084166
e-ISSN	14803275
Journal	Canadian Journal of Microbiology
Issue Number	8
Volume Number	58
Language	English
Publisher	Canadian Science Publishing
Publisher Date	2012-08-01
Publisher Place	Canada
Access Restriction	Open
Subject Keyword	Discipline Microbiology Antifungal Agents Chemistry Lipopeptides Pharmacology Amino Acid Sequence Animals Anti-infective Agents Bacillus Drug Effects Caenorhabditis Elegans Larva Mass Spectrometry Pseudomonas Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology Immunology Microbiology Applied Microbiology and Biotechnology

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