NDLI: Brassica napus polygalacturonase inhibitor proteins inhibit Sclerotinia sclerotiorum polygalacturonase enzymatic and necrotizing activities and delay symptoms in transgenic plants.

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Brassica napus polygalacturonase inhibitor proteins inhibit Sclerotinia sclerotiorum polygalacturonase enzymatic and necrotizing activities and delay symptoms in transgenic plants.

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Year: 2013 Volume: 59 Issue: 1

Brassica napus polygalacturonase inhibitor proteins inhibit Sclerotinia sclerotiorum polygalacturonase enzymatic and necrotizing activities and delay symptoms in transgenic plants.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bashi, Zafer Dallal Rimmer, S. Roger Khachatourians, George G. Hegedus, Dwayne D.
Description	Country affiliation: Canada Author Affiliation: Bashi ZD ( Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada.)
Abstract	Sclerotinia sclerotiorum releases a battery of polygalacturonases (PGs) during infection, which the host plant may cope with through production of polygalacturonase inhibitor proteins (PGIPs). To study the interaction between S. sclerotiorum PGs and Brassica napus PGIPs, 5 S. sclerotiorum PGs and 4 B. napus PGIPs were expressed in Pichia pastoris. SsPG3, SsPG6, and BnPGIP1 were successfully produced in the yeast system, and BnPGIP1 inhibited SsPG6 enzymatic activity in vitro. SsPG3 and SsPG6 both induced light-dependent necrosis when infiltrated into leaves, which was reduced in an Arabidopsis thaliana line expressing BnPGIP2 and to a lesser extent in a line expressing BnPGIP1. The line expressing BnPGIP2 also exhibited a delay in the onset of symptoms upon S. sclerotiorum inoculation, but no long-term effect on S. sclerotiorum disease progression was observed. The P. pastoris system was found to be suitable for expressing high levels of some S. sclerotiorum PGs, but PGIP interaction studies were best performed in planta. Arabidopsis thaliana forms necrotic lesions upon infiltration of PGs, is susceptible to S. sclerotiorum, and is easily transformed, and thus, is well-suited for the qualitative study of PG-PGIP interactions.
File Format	HTM / HTML
ISSN	00084166
e-ISSN	14803275
Journal	Canadian Journal of Microbiology
Issue Number	2
Volume Number	59
Language	English
Publisher	Canadian Science Publishing
Publisher Date	2013-02-01
Publisher Place	Canada
Access Restriction	Open
Subject Keyword	Discipline Microbiology Ascomycota Enzymology Brassica Napus Genetics Microbiology Enzyme Inhibitors Metabolism Plant Proteins Arabidopsis Gene Expression Plants, Genetically Modified Polygalacturonase Antagonists & Inhibitors Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology Immunology Microbiology Applied Microbiology and Biotechnology

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