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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bhattachariya, A. Dahan, D. Ekman, M. Boettger, T. Braun, T. Swärd, K. Hellstrand, P. Albinsson, S. |
| Description | Country affiliation: Sweden Author Affiliation: Bhattachariya A ( Department of Experimental Medical Science, Lund University, Lund, Sweden.); Dahan D ( Department of Experimental Medical Science, Lund University, Lund, Sweden.); Ekman M ( Department of Experimental Medical Science, Lund University, Lund, Sweden.); Boettger T ( Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.); Braun T ( Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.); Swärd K ( Department of Experimental Medical Science, Lund University, Lund, Sweden.); Hellstrand P ( Department of Experimental Medical Science, Lund University, Lund, Sweden.); Albinsson S ( Department of Experimental Medical Science, Lund University, Lund, Sweden.) |
| Abstract | AIM: Stretch is essential for maintaining the contractile phenotype of vascular smooth muscle cells, and small non-coding microRNAs are known to be important in this process. Using a Dicer knockout model, we have previously reported that microRNAs are essential for stretch-induced differentiation and regulation of L-type calcium channel expression. The aim of this study was to investigate the importance of the smooth muscle-enriched miR-143/145 microRNA cluster for stretch-induced differentiation of the portal vein. METHODS: Contractile force and depolarization-induced calcium influx were determined in portal veins from wild-type and miR-143/145 knockout mice. Stretch-induced contractile differentiation was investigated by determination of mRNA expression following organ culture for 24 h under longitudinal load by a hanging weight. RESULTS: In the absence of miR-143/145, stretch-induced mRNA expression of contractile markers in the portal vein was reduced. This was associated with decreased amplitude of spontaneous activity and depolarization-induced contractile and intracellular calcium responses, while contractile responses to 5-HT were largely maintained. We found that these effects correlated with a reduced basal expression of the pore-forming subunit of L-type calcium channels and an increased expression of CaMKIIδ and the transcriptional repressor DREAM. CONCLUSION: Our results suggest that the microRNA-143/145 cluster plays a role in maintaining stretch-induced contractile differentiation and calcium signalling in the portal vein. This may have important implications for the use of these microRNAs as therapeutic targets in vascular disease. |
| File Format | HTM / HTML |
| ISSN | 17481708 |
| Issue Number | 3 |
| Volume Number | 215 |
| e-ISSN | 17481716 |
| Journal | Acta Physiologica |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2015-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Physiology Cell Differentiation Genetics Mechanotransduction, Cellular Micrornas Muscle, Smooth, Vascular Metabolism Animals Blotting, Western Mice Mice, Knockout Muscle Contraction Myocytes, Smooth Muscle Organ Culture Techniques Portal Vein Real-time Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology |
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