NDLI: Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA.

Please wait, while we are loading the content...

Year: 2017 Volume: 19

Year: 2016 Volume: 18

Year: 2015 Volume: 17

Year: 2014 Volume: 16

Year: 2013 Volume: 15

Year: 2012 Volume: 14

Year: 2011 Volume: 13

Year: 2010 Volume: 12

Year: 2009 Volume: 11

Year: 2008 Volume: 10

Year: 2007 Volume: 9

Year: 2006 Volume: 8

Year: 2005 Volume: 7

Year: 2004 Volume: 6

Year: 2003 Volume: 5

Year: 2002 Volume: 4

Year: 2001 Volume: 3

Year: 2000 Volume: 2

Year: 2000 Volume: 2 Issue: 4

Year: 2000 Volume: 2 Issue: 3

Angiotensin I-converting enzyme: genotype and disease associations.

The development of a multitarget, multicolor fluorescence in situ hybridization assay for the detection of urothelial carcinoma in urine.

Reproducibility of alternative probe synthesis approaches for gene expression profiling with arrays.

Novel polymorphism in the FMR1 gene resulting in a 'pseudodeletion' of FMR1 in a commonly used fragile X assay.

Specificity of TLS-CHOP rearrangement for classic myxoid/round cell liposarcoma: absence in predominantly myxoid well-differentiated liposarcomas.

Improving the detection of p53 mutations in breast cancer by use of the FASAY, a functional assay.

Pseudo-spikes are common in histologically benign lymphoid tissues.

Analytical evaluation of primer engineered multiplex polymerase chain reaction-restriction fragment length polymorphism for detection of factor V Leiden and prothrombin G20210A.

Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA.

Highlights of the Ninth Annual Beaumont Symposium on Molecular Pathology: DNA technology in the clinical laboratory.

Year: 2000 Volume: 2 Issue: 2

Year: 2000 Volume: 2 Issue: 1

Year: 1999 Volume: 1

Performance characteristics of a quantitative, homogeneous TaqMan RT-PCR test for HCV RNA.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kleiber, J. Walter, T. Haberhausen, G. Tsang, S. Babiel, R. Rosenstraus, M.
Description	Country affiliation: United States Author Affiliation: Kleiber J ( Roche Molecular Systems, Inc., Pleasanton, California, USA.)
Abstract	We developed a homogeneous format reverse transcription-polymerase chain reaction assay for quantitating hepatitis C virus (HCV) RNA based on the TaqMan principle, in which signal is generated by cleaving a target-specific probe during amplification. The test uses two probes, one specific for HCV and one specific for an internal control, containing fluorophores with different emission spectra. Titers are calculated in international units (IU)/ml by comparing the HCV signal generated by test samples to that generated by a set of external standards. Endpoint titration experiments demonstrated that samples containing 28 IU/ml give positive results 95% of the time. Based on these data, the limit of detection was set conservatively at 40 IU/ml. All HCV genotypes were amplified with equal efficiency and accurately quantitated: when equal quantities of RNA were tested, each genotype produced virtually identical fluorescent signals. The test exhibited a linear range extending from 64 to 4,180,000 IU/ml and excellent reproducibility, with coefficients of variation ranging from 21.6 to 30.4%, which implies that titers that differ by a factor of twofold (0.3 log10) are statistically significant (P = 0.005). The test did not react with other organisms likely to co-infect patients with hepatitis C and exhibited a specificity of 99% when evaluated on a set of samples from HCV seronegative blood donors. In interferon-treated patients, the patterns of viral load changes revealed by the TaqMan HCV quantitative test distinguished responders from nonresponders and responder-relapsers. These data indicate that the TaqMan quantitative HCV test provides an attractive alternative for measuring HCV viral load and should prove useful for prognosis and for monitoring the efficacy of antiviral treatments.
File Format	HTM / HTML
ISSN	15251578
e-ISSN	19437811
Journal	The Journal of Molecular Diagnostics
Issue Number	3
Volume Number	2
Language	English
Publisher	Elsevier
Publisher Date	2000-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Hepacivirus Genetics Hepatitis C Virology Rna, Viral Blood Reverse Transcriptase Polymerase Chain Reaction Taq Polymerase Metabolism Genotype Growth & Development Diagnosis Drug Therapy Prognosis Reference Standards Reproducibility Of Results Sensitivity And Specificity Viral Load
Content Type	Text
Resource Type	Article
Subject	Molecular Medicine Pathology and Forensic Medicine

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in