NDLI: Custom design of a GeXP multiplexed assay used to assess expression profiles of inflammatory gene targets in normal colon, polyp, and tumor tissue.

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Custom design of a GeXP multiplexed assay used to assess expression profiles of inflammatory gene targets in normal colon, polyp, and tumor tissue.

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Year: 2011 Volume: 13 Issue: 1

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Custom design of a GeXP multiplexed assay used to assess expression profiles of inflammatory gene targets in normal colon, polyp, and tumor tissue.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Drew, Janice E. Mayer, Claus-Dieter Farquharson, Andrew J. Young, Pauline Barrera, Lawrence N.
Description	Country affiliation: United kingdom Author Affiliation: Drew JE ( Division of Gut Health, Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, Scotland. j.drew@abdn.ac.uk)
Abstract	Colon cancers are characterized by aberrant gene expression signatures associated with disease initiation and progression. Identification of aberrant gene expression associated with colon carcinogenesis has increased significantly with application of gene array technologies. Downstream processing of these data has been hindered by the lack of robust multiplexed gene quantitative technologies facilitating study of the identified multiple gene targets. The GenomeLab Genetic Analysis System presents a novel technology platform for quantitative multiplexed gene expression analysis. This report describes the custom design of a GeXP multiplexed assay used to assess expression profiles of 14 inflammatory gene targets in normal, polyp, and tumor tissue. Characteristic normal, polyp, and tumor tissue gene expression profiles were obtained. Statistical analysis confirmed comparable relative quantitation of gene expression using the GeXP, macroarray, and single-plex real-time polymerase chain reaction assays. GeXP assays may be usefully applied in clinical and regulatory studies of multiple gene targets. This system permits custom-design options for relative quantification of multiple gene target expression, simultaneously in a single reaction, using nanogram quantities of total RNA template. The system provides an approach to advance the study of multiple targets identified from gene array analysis with potential for characterizing gene expression signatures in clinical diagnostics.
File Format	HTM / HTML
ISSN	15251578
e-ISSN	19437811
DOI	10.1016/j.jmoldx.2010.10.001
Journal	The Journal of Molecular Diagnostics
Issue Number	2
Volume Number	13
Language	English
Publisher	Elsevier
Publisher Date	2011-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Colonic Neoplasms Genetics Colonic Polyps Gene Expression Profiling Neoplasms Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Tumor Markers, Biological Biopsy Pathology Inflammation Evaluation Studies Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Medicine Pathology and Forensic Medicine

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Custom design of a GeXP multiplexed assay used to assess expression profiles of inflammatory gene targets in normal colon, polyp, and tumor tissue.

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