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Year: 2012 Volume: 14 Issue: 1

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Impact of collection and storage of lung tumor tissue on whole genome expression profiling.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Freidin, Maxim B. Bhudia, Neesa Lim, Eric Nicholson, Andrew G. Cookson, William O. Moffatt, Miriam F.
Description	Country affiliation: United kingdom Author Affiliation: Freidin MB ( National Heart and Lung Institute, Imperial College London, London, United Kingdom.)
Abstract	Gene expression profiling could assist in revealing biomarkers of lung cancer prognosis and progression. The handling of biological samples may strongly influence global gene expression, a fact that has not been addressed in many studies. We sought to investigate the changes in gene expression that may occur as a result of sample processing time and conditions. Using Illumina Human WG-6 arrays, we quantified gene expression in lung carcinoma samples from six patients obtained at chest opening before and immediately after lung resection with storage in RNAlater [T1a((CO)) and T1b((LR))], after receipt of the sample for histopathology, placed in RNAlater [T2a((HP))]; snap frozen [T2b((HP.SF))]; or snap frozen and stored for 1 week [T2c((HP.SFA))], as well as formalin-fixed, paraffin-embedded (FFPE) block samples. Sampling immediately after resection closely represented the tissue obtained in situ, with only 1% of genes differing more than twofold [T1a((CO)) versus T1b((LR))]. Delaying tissue harvest for an average of 30 minutes from the operating theater had a significant impact on gene expression, with approximately 25% of genes differing between T1a((CO)) and T2a((HP)). Many genes previously identified as lung cancer biomarkers were altered during this period. Examination of FFPE specimens showed minimal correlation with fresh samples. This study shows that tissue collection immediately after lung resection with conservation in RNAlater is an optimal strategy for gene expression profiling.
File Format	HTM / HTML
ISSN	15251578
e-ISSN	19437811
DOI	10.1016/j.jmoldx.2011.11.002
Journal	The Journal of Molecular Diagnostics
Issue Number	2
Volume Number	14
Language	English
Publisher	Elsevier
Publisher Date	2012-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Tumor Markers, Biological Genetics Carcinoma, Non-small-cell Lung Classification Gene Expression Profiling Specimen Handling Adenocarcinoma Pathology Adenocarcinoma, Bronchiolo-alveolar Metabolism Carcinoma, Large Cell Carcinoma, Squamous Cell Lung Neoplasms Oligonucleotide Array Sequence Analysis Paraffin Embedding Tissue Banks Tissue Fixation Comparative Study
Content Type	Text
Resource Type	Article
Subject	Molecular Medicine Pathology and Forensic Medicine

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