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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Vondráková, Zuzana Eliásová, Katerina Vágner, Martin |
| Description | Country affiliation: Czech Republic Author Affiliation: Vondráková Z ( Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-16502 Prague 6, Czech Republic.); Eliásová K ( Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-16502 Prague 6, Czech Republic.); Vágner M ( Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-16502 Prague 6, Czech Republic. Electronic address: vagner@ueb.cas.cz.) |
| Abstract | The role of the actin cytoskeleton in somatic embryo development was investigated using latrunculin B and cytochalasin D. Brief treatments (1h) with either drug at the start of maturation fragmented the actin in suspensor cells and/or depolymerized actin filaments in meristematic cells. The drugs targeted different cells: latB primarily affected the suspensor cells, but cchD damaged both suspensor and meristematic cells. Lethal damage to the meristematic and suspensor cells was observed when the drugs were applied throughout the maturation period, although the severity of this effect depended on their concentrations. The drugs' effects on the yield of mature somatic embryos were investigated by applying them to embryo cultures throughout the maturation period or for one week at three different points in the maturation process: immediately prior to the start of maturation, during the first week of maturation, and during the fourth week of maturation. The strongest effects were observed when the drugs were applied at the start of maturation. Under these conditions, latB destroyed the suspensors, eliminating the underdeveloped embryos that depend on them. This accelerated the development of embryos that were capable of separating from the suspensors. Thus, while the total number of embryos at the end of the maturation period was lower than in untreated control cultures, the surviving mature embryos were of high quality. cchD treatment at the start of maturation strongly inhibited embryo development. Drug treatment at the end of the maturation period did not significantly affect embryo development: latB caused no change in the yield of somatic embryos, but cchD treatment increased the number of malformed embryos compared to untreated controls. |
| File Format | HTM / HTML |
| ISSN | 01689452 |
| Volume Number | 221-222 |
| e-ISSN | 18732259 |
| Journal | Plant Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-05-01 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Botany Actins Antagonists & Inhibitors Bicyclo Compounds, Heterocyclic Pharmacology Cytochalasin D Nucleic Acid Synthesis Inhibitors Picea Drug Effects Plant Somatic Embryogenesis Techniques Thiazolidines Metabolism Microscopy, Confocal Administration & Dosage Embryology Seeds Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Medicine Agronomy and Crop Science |
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