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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yang, Liang Wu, Kangcheng Gao, Peng Liu, Xiaojuan Li, Guangpu Wu, Zujian |
| Description | Country affiliation: China Author Affiliation: Yang L ( Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China.); Wu K ( Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China.); Gao P ( College of Horticulture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.); Liu X ( Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China.); Li G ( Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.); Wu Z ( Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian, China. Electronic address: wuzujian@126.com.) |
| Abstract | Plant LRR-RLKs serve as protein interaction platforms, and as regulatory modules of protein activation. Here, we report the isolation of a novel plant-specific LRR-RLK from Glycine soja (termed GsLRPK) by differential screening. GsLRPK expression was cold-inducible and shows Ser/Thr protein kinase activity. Subcellular localization studies using GFP fusion protein indicated that GsLRPK is localized in the plasma membrane. Real-time PCR analysis indicated that temperature, salt, drought, and ABA treatment can alter GsLRPK gene transcription in G. soja. However, just protein induced by cold stress not by salinity and ABA treatment in tobacco was found to possess kinase activity. Furthermore, we found that overexpression of GsLRPK in yeast and Arabidopsis can enhance resistance to cold stress and increase the expression of a number of cold responsive gene markers. |
| File Format | HTM / HTML |
| ISSN | 01689452 |
| Volume Number | 215-216 |
| e-ISSN | 18732259 |
| Journal | Plant Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-02-01 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Botany Adaptation, Physiological Genetics Cold Temperature Genes, Plant Membrane Proteins Soybean Proteins Soybeans Stress, Physiological Abscisic Acid Pharmacology Amino Acid Sequence Arabidopsis Metabolism Cell Membrane Droughts Gene Expression Regulation, Plant Leucine Molecular Sequence Data Protein Kinases Real-time Polymerase Chain Reaction Salt-tolerance Drug Effects Tobacco Transcription, Genetic Yeasts Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Medicine Agronomy and Crop Science |
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