| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Di Tomo, P. Di Silvestre, S. Cordone, V. G. P. Giardinelli, A. Faricelli, B. Pipino, C. Lanuti, P. Peng, T. Formoso, G. Yang, D. Arduini, A. Chiarelli, F. Pandolfi, A. Di Pietro, N. |
| Description |
Country affiliation: Italy Author Affiliation: Di Tomo P ( Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti, Italy); Di Silvestre S ( Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti, Italy); Cordone VG ( Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti, Italy); Giardinelli A ( Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti, Italy); Faricelli B ( Department of Medicine and Aging Sciences, 'G. d'Annunzio' University, Chieti, Italy); Pipino C ( Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti, Italy); Lanuti P ( Department of Medicine and Aging Sciences, 'G. d'Annunzio' University, Chieti, Italy); Peng T ( Morningside Laboratory for Chemical Biology and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.); Formoso G ( Department of Medicine and Aging Sciences, 'G. d'Annunzio' University, Chieti, Italy); Yang D ( Morningside Laboratory for Chemical Biology and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China.); Arduini A ( CoreQuest Sagl, Manno, Ticino, Switzerland.); Chiarelli F ( Department of Medicine and Aging Sciences, 'G. d'Annunzio' University, Chieti, Italy.); Pandolfi A ( Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti, Italy); Di Pietro N ( Department of Medical, Oral and Biotechnological Sciences, 'G. d'Annunzio' University, Chieti, Italy) |
| Abstract |
BACKGROUND AND AIMS: Diabetes mellitus is associated with inflammatory endothelial activation and increased vascular leukocyte adhesion molecule expression, both playing a prominent role in the development of vascular complications. Centella asiatica (CA) and Lipoic Acid (LA) have shown anti-inflammatory and anti-oxidant properties in a variety of experimental models; however, their action on human umbilical vein endothelial cells (HUVECs), chronically exposed to hyperglycemia and pro-inflammatory environment during pregnancy, is still unknown. METHODS AND RESULTS: In HUVECs from umbilical cords of gestational diabetic (GD) or healthy (C) women, both CA and LA affected tumor necrosis factor- (TNF- )-induced inflammation, being associated with a significant decrease in vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) expression (western blot) and exposure (flow cytometry), as well as monocyte-HUVECs interaction (adhesion assay). Notably, this was associated with a significant reduction of an index of nitro-oxidative stress, such as the intracellular peroxynitrite levels (fluorescence detection by cytometric analysis), Mitogen-Activated Protein kinase (p44/42 MAPK) expression/phosphorylation levels and Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB p65) cytoplasm-nucleus translocation (flow cytometry). Overall our results indicate that both CA and LA used separately, and even better when combined, are effective to reduce the inflammatory response in TNF- -treated HUVECs. Notably, this was more significant in GD than in C-HUVECs and also evident at baseline. CONCLUSION: In conclusion, our in vitro study demonstrates that both CA and LA, or a combination thereof, are able to mitigate the potentially dangerous effects on the endothelium of chronic exposure to hyperglycemia in vivo. |
| File Format |
HTM / HTML |
| ISSN |
09394753 |
| Issue Number |
7 |
| Volume Number |
25 |
| e-ISSN |
15903729 |
| Journal |
Nutrition, Metabolism and Cardiovascular Diseases |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-07-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Metabolism Discipline Cardiology Discipline Vascular Diseases Discipline Nutritional Sciences Antioxidants Pharmacology Cell Adhesion Drug Effects Diabetes, Gestational Pathology Endothelial Cells Human Umbilical Vein Endothelial Cells Monocytes Thioctic Acid Triterpenes Adult Cell Adhesion Molecules Biosynthesis Female Humans Pregnancy Signal Transduction Tumor Necrosis Factor-alpha Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Nutrition and Dietetics Endocrinology, Diabetes and Metabolism Cardiology and Cardiovascular Medicine |
