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Label-Free Direct Electronic Detection of Biomolecules with Amorphous Silicon Nanostructures
| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lund, John Mehta, Ranjana Parviz, Babak A. |
| Description | Country affiliation: United States Author Affiliation: Lund J ( Department of Electrical Engineering, University of Washington, Seattle, Washington 98195-2500, USA.) |
| Abstract | We present the fabrication and characterization of a nano-scale sensor made of amorphous silicon for the label-free, electronic detection of three classes of biologically important molecules: ions, oligonucleotides, and proteins. The sensor structure has an active element which is a 50 nm wide amorphous silicon semicircle and has a total footprint of less than 4 $μm^{2}.$ We demonstrate the functionalization of the sensor with receptor molecules and the electronic detection of three targets: $H^{+}$ ions, short single-stranded DNAs, and streptavidin. The sensor is able to reliably distinguish single base-pair mismatches in 12 base long strands of DNA and monitor the introduction and identification of straptavidin in real-time. The versatile sensor structure can be readily functionalized with a wide range of receptor molecules and is suitable for integration with high-speed electronic circuits as a post-process on an integrated circuit chip. |
| File Format | HTM / HTML |
| ISSN | 15499634 |
| e-ISSN | 15499642 |
| Journal | Nanomedicine: Nanotechnology, Biology and Medicine |
| Issue Number | 4 |
| Volume Number | 2 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2006-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Nanotechnology Discipline Biotechnology Biosensing Techniques Metal Nanoparticles Chemistry Nanotechnology Silicon Biological Markers Electrons Equipment Design Ions Materials Testing Nanostructures Oligonucleotide Array Sequence Analysis Oligonucleotides Streptavidin |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Molecular Medicine Bioengineering Biomedical Engineering Pharmaceutical Science |
