NDLI: In-Depth Fluorescence Lifetime Imaging Analysis Revealing SNAP25A-Rabphilin 3A Interactions

Please wait, while we are loading the content...

Year: 2017 Volume: 23

Year: 2016 Volume: 22

Year: 2015 Volume: 21

Year: 2014 Volume: 20

Year: 2013 Volume: 19

Year: 2012 Volume: 18

Year: 2011 Volume: 17

Year: 2010 Volume: 16

Year: 2009 Volume: 15

Year: 2008 Volume: 14

Year: 2008 Volume: 14 Issue: 6

Seeing the brain in action: how multiphoton imaging has advanced our understanding of neuronal function.

Cardiovascular imaging using two-photon microscopy.

In-Depth Fluorescence Lifetime Imaging Analysis Revealing SNAP25A-Rabphilin 3A Interactions

Two-photon imaging of cellular activities in oxygen sensing tissues.

Second-harmonic generation imaging of membrane potential with photon counting.

Nonlinear optical imaging of cellular processes in breast cancer.

A method for chronological intravital imaging of bovine oocytes during in vitro maturation.

Chromatic aberrations in the field evaporation behavior of small precipitates.

On the Field Evaporation Behavior of a Model Ni-Al-Cr Superalloy Studied by Picosecond Pulsed-Laser Atom-Probe Tomography

Year: 2008 Volume: 14 Issue: 5

Year: 2008 Volume: 14 Issue: 4

Year: 2008 Volume: 14 Issue: 3

Year: 2008 Volume: 14 Issue: 2

Year: 2008 Volume: 14 Issue: Supplementary 2

Year: 2008 Volume: 14 Issue: 1

Year: 1998 Volume: 4

In-Depth Fluorescence Lifetime Imaging Analysis Revealing SNAP25A-Rabphilin 3A Interactions

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Jiung-De Huang, Ping-Chun Lin, Yi-Cheng Kao, Lung-Sen Huang, Chien-Chang Kao, Fu-Jen Lin, Chung-Chih Yang, De-Ming
Description	Country affiliation: China Author Affiliation: Lee JD ( Department of Medical Research and Education, Taipei Veterans General Hospital, National Yang-Ming University, Taipei 11217, Taiwan, Republic of China.)
Abstract	Abstract The high sensitivity and spatial resolution enabled by two-photon excitation fluorescence lifetime imaging microscopy/fluorescence resonance energy transfer (2PE-FLIM/FRET) provide an effective approach that reveals protein-protein interactions in a single cell during stimulated exocytosis. Enhanced green fluorescence protein (EGFP)–labeled synaptosomal associated protein of 25 kDa (SNAP25A) and red fluorescence protein (mRFP)–labeled Rabphillin 3A (RPH3A) were co-expressed in PC12 cells as the FRET donor and acceptor, respectively. The FLIM images of EGFP-SNAP25A suggested that SNAP25A/RPH3A interaction was increased during exocytosis. In addition, the multidimensional (three-dimensional with time) nature of the 2PE-FLIM image datasets can also resolve the protein interactions in the z direction, and we have compared several image analysis methods to extract more accurate and detailed information from the FLIM images. Fluorescence lifetime was fitted by using one and two component analysis. The lifetime FRET efficiency was calculated by the peak lifetime (τpeak) and the left side of the half-peak width (τ1/2), respectively. The results show that FRET efficiency increased at cell surface, which suggests that SNAP25A/RPH3A interactions take place at cell surface during stimulated exocytosis. In summary, we have demonstrated that the 2PE-FLIM/FRET technique is a powerful tool to reveal dynamic SNAP25A/RPH3A interactions in single neuroendocrine cells.
File Format	HTM / HTML
ISSN	14319276
Issue Number	6
Volume Number	14
e-ISSN	14358115
Journal	Microscopy and Microanalysis
Language	English
Publisher	Cambridge University Press
Publisher Date	2008-12-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Chemistry Techniques Adaptor Proteins, Signal Transducing Metabolism Microscopy, Fluorescence, Multiphoton Nerve Tissue Proteins Synaptosomal-associated Protein 25 Vesicular Transport Proteins Genetics Animals Cell Line Exocytosis Fluorescence Resonance Energy Transfer Image Processing, Computer-assisted Luminescent Proteins Neuroendocrine Cells Cytology Protein Binding Protein Transport Rats Recombinant Fusion Proteins Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Instrumentation

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in