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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chokechanachaisakul, Uraiwan Kaneko, Tomoatsu Yamanaka, Yusuke Okiji, Takashi Suda, Hideaki |
| Description | Country affiliation: Japan Author Affiliation: Chokechanachaisakul U ( Department of Pulp Biology and Endodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.) |
| Abstract | In conventional whole-tooth culture systems, limitation exists regarding maintenance of the vitality of the dental pulp, because this tissue is encased in rigid dentin walls that hinder nutrition supply. We here report a whole tooth-in-jaw-bone culture system of rat mandibular first molars, where transcardiac perfusion with culture medium was carried out before placement of the jaw bone into culture medium, aiming to facilitate longer time preservation of the dental pulp tissue. Following 7 days of culture, the pulp tissues were analyzed by histology and immunohistochemistry to ED2 (antiresident macrophage). ED2-positive macrophages were also analyzed for their Class II MHC, interleukin-6 (IL-6), and p53 mRNA expression levels by means of immune-laser capture microdissection (immune-LCM). Dentin sialophosphoprotein (DSPP) mRNA expression in odontobalstic layer was also examined by LCM. Teeth cultured following saline-perfusion and nonperfusion served as cultured controls. Normal teeth also served as noncultured controls. Histological examination demonstrated that the structure of the pulp tissue was well preserved in the medium-perfused explants in contrast to the cultured control groups. The Class II MHC, IL-6, and p53 mRNA expression levels of ED2-positive cells and DSPP expression levels of odontoblastic layer tissues in the pulp of medium-perfused explants were not significantly different from those in the noncultured normal teeth. In conclusion, the structural integrity and mRNA expression in the pulp were maintained at the in vivo level in the ex vivo whole tooth-in-jaw-bone culture system. The system may lay the foundation for studies aiming at defining further histological and molecular mechanism of the pulp. |
| File Format | HTM / HTML |
| ISSN | 1059910X |
| Issue Number | 10 |
| Volume Number | 75 |
| e-ISSN | 10970029 |
| Journal | Microscopy Research and Technique |
| Language | English |
| Publisher | Wiley-Liss |
| Publisher Date | 2012-10-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Chemistry Techniques Jaw Physiology Molar Animals Culture Media Chemistry Dental Pulp Pathology Gene Expression Profiling Immunohistochemistry Laser Capture Microdissection Anatomy & Histology Organ Culture Techniques Methods Rats Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Anatomy Histology Instrumentation Medical Laboratory Technology |
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