NDLI: Study of the oipA genetic diversity and EPIYA motif patterns in cagA-positive Helicobacter pylori strains from Venezuelan patients with chronic gastritis.

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Study of the oipA genetic diversity and EPIYA motif patterns in cagA-positive Helicobacter pylori strains from Venezuelan patients with chronic gastritis.

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Study of the oipA genetic diversity and EPIYA motif patterns in cagA-positive Helicobacter pylori strains from Venezuelan patients with chronic gastritis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Torres, Keila Valderrama, Elvis Sayegh, Marjorie Ramírez, José Luis Chiurillo, Miguel Angel
Spatial Coverage	Venezuela
Description	Author Affiliation: Torres K ( Laboratorio de Genética Molecular 'Dr. Jorge Yunis-Turbay', Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela. Electronic address: keilatorres@ucla.edu.ve.); Valderrama E ( Departamento de Anatomía Patológica, Hospital Antonio María Pineda-UCLA, Barquisimeto, Venezuela. Electronic address: elvisvalderrama@hotmail.com.); Sayegh M ( Centro de Biotecnología, Instituto de Estudios Avanzados, Caracas, Venezuela. Electronic address: marjo.say@gmail.com.); Ramírez JL ( Centro de Biotecnología, Instituto de Estudios Avanzados, Caracas, Venezuela. Electronic address: ramjoseluis@gmail.com.); Chiurillo MA ( Laboratorio de Genética Molecular 'Dr. Jorge Yunis-Turbay', Decanato de Ciencias de la Salud, Universidad Centroccidental Lisandro Alvarado (UCLA), Barquisimeto, Venezuela. Electronic address: mchiurillo@ucla.edu.ve.)
Abstract	CagA and OipA are involved, among other virulence factors, in the ability of Helicobacter pylori to colonize the gastric mucosa and to modulate the host environment during the establishment of chronic infection. The number and type of EPIYA phosphorylation motifs and the presence and functional status of oipA have been involved in the induction of cellular transformations playing an important role in the development of H. pylori associated gastric diseases. This work determined the prevalence of the oipA virulence factor and EPIYA motif patterns in cagA-positive H. pylori gastric biopsies from chronic gastritis patients from the Central-Western region of Venezuela. DNA was extracted directly from gastric biopsies collected by upper endoscopy from 113 patients. The EPIYA motif genotyping and oipA gene functional status was determined by PCR and sequencing. Phylogenetic analysis with the 3' variable region of cagA sequences was performed. Only Western-type EPIYA variants were detected: ABC (68.14%), ABCC (29.20%) and ABCCC (2.66%). High prevalence of strains with the oipA gene (93.8%) and its functional status 'ON' (83%) was observed. No significant association between EPIYA motif patterns or oipA functional status with the histological changes in the gastric mucosa was found. Our study demonstrated the absolute predominance of the Western-type cagA gene in a Venezuelan admixed population. This is the first report showing oipA status of H. pylori strains in Venezuela. Further studies with a larger number of samples and including other pathologies are necessary to continue evaluating the role of the H. pylori virulence factors in the prevalence of gastric diseases in our country.
File Format	HTM / HTML
ISSN	08824010
Volume Number	76
e-ISSN	10961208
Journal	Microbial Pathogenesis
Language	English
Publisher	Elsevier
Publisher Date	2014-11-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Antigens, Bacterial Genetics Bacterial Outer Membrane Proteins Bacterial Proteins Gastritis Microbiology Genetic Variation Helicobacter Infections Helicobacter Pylori Classification Amino Acid Motifs Biopsy Chronic Disease Cluster Analysis Dna, Bacterial Chemistry Gastric Mucosa Pathology Genotype Isolation & Purification Histocytochemistry Humans Molecular Sequence Data Polymerase Chain Reaction Sequence Analysis, Dna Sequence Homology Venezuela Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology

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