NDLI: Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections.

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Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Brillowska-Dabrowska, Anna Swierkowska, Aleksandra Lindhardt Saunte, Ditte Marie Arendrup, Maiken Cavling
Description	Country affiliation: Denmark Author Affiliation: Brillowska-Dabrowska A ( Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark. abrill@chem.pg.gda.pl)
Abstract	Since traditional diagnosis of dermatophyte infections is slow, we present a rapid new PCR test for detection of Trichophyton spp., Microsporum canis and M. audouinii infections. The performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human DNA control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected with M. canis; and two samples from un-infected control animals. DNA was prepared by a 10-min procedure from pure cultures as previously described. The 302 bp PCR product was obtained for 35/35 Trichophyton isolates (10 species included) and the 279 bp for 3/3 M. canis and 4/4 M. audouinii samples. None of the 2 E. floccosum, 11 M. gypseum, 3 M M. persicolor or 12 control samples (yeast, mould, human DNA) were positive with either of the two PCR tests. Among the patient specimens, seven were T. rubrum positive, two for T. mentagrophytes, one was positive for T. tonsurans and 15 were dermatophyte negative by routine investigation (culture and/or pan-dermatophyte + T. rubrum multiplex PCR). The PCR results with our procedures were in 100% agreement with these results. Finally, the Microsporum PCR was positive for 10/10 guinea pig specimens from infected animals but for 0/2 of the control animal samples. The evaluation of the two PCR tests indicated excellent sensitivity and specificity.
File Format	HTM / HTML
ISSN	13693786
e-ISSN	14602709
Journal	Medical Mycology
Issue Number	3
Volume Number	48
Language	English
Publisher	Oxford University Press
Publisher Date	2010-05-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Veterinary Medicine Dermatomycoses Diagnosis Microbiology Microsporum Classification Isolation & Purification Polymerase Chain Reaction Trichophyton Animals Dna, Fungal Genetics Guinea Pigs Sensitivity And Specificity Comparative Study Evaluation Studies
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary

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