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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fernandes, Jane Luiza Nogueira de Souza, Rodrigo Octavio Mendonça Alves de Vasconcellos Azeredo, Rodrigo Bagueira |
| Description | Country affiliation: Brazil Author Affiliation: Fernandes JL ( Laboratório de Aplicações da RMN, Departamento de Química Orgânica, Instituto de Química, Universidade Federal Fluminense, Rua Outeiro de São João Batista s/n°, CEP: 24020-150, Niterói, Rio de Janeiro, Brazil.) |
| Abstract | In the present investigation, we studied the enzymatic synthesis of monoacylglycerols (MAG) and diacylglycerols (DAG) via the esterification of saturated fatty acids (stearic, palmitic and an industrial residue containing 87% palmitic acid) and glycerol in a solvent-free system. Three immobilized lipases (Lipozyme RM IM, Lipozyme TL IM and Novozym 435) and different reaction conditions were evaluated. Under the optimal reaction conditions, esterifications catalyzed by Lipozyme RM IM resulted in a mixture of MAG and DAG at high conversion rates for all of the substrates. In addition, except for the reaction of industrial residue at atmospheric pressure, all of these products met the World Health Organization and European Union directives for acylglycerol mixtures for use in food applications. The products were quantified by (13)C NMR, with the aid of an external reference signal which was generated from a sealed coaxial tube filled with acetonitrile-d3. After calibrating the area of this signal using the classical external reference method, the same coaxial tube was used repeatedly to quantify the reaction products. |
| File Format | HTM / HTML |
| ISSN | 07491581 |
| Issue Number | 6 |
| Volume Number | 50 |
| e-ISSN | 1097458X |
| Journal | Magnetic Resonance in Chemistry |
| Language | English |
| Publisher | Wiley Heyden |
| Publisher Date | 2012-06-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Chemistry Diglycerides Analysis Fatty Acids Chemistry Lipase Magnetic Resonance Spectroscopy Methods Monoglycerides Calibration Carbon Isotopes Catalysis Chemical Synthesis Esterification Reference Standards Solvents Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Materials Science |
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