NDLI: X-ray micro-diffraction studies on biological samples at the BioCAT Beamline 18-ID at the Advanced Photon Source.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Barrea, R. A. Antipova, O. Gore, D. Heurich, R. Vukonich, M. Kujala, N. G. Irving, T. C. Orgel, J. P. R. O.
Spatial Coverage	Illinois
Description	Country affiliation: United States Author Affiliation: Barrea RA ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.); Antipova O ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.); Gore D ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.); Heurich R ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.); Vukonich M ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.); Kujala NG ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.); Irving TC ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.); Orgel JP ( The Biophysics Collaborative Access Team (BioCAT), CSRRI and Departments of Biology, Physics and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL 60616, USA.)
Abstract	The small source sizes of third-generation synchrotron sources are ideal for the production of microbeams for diffraction studies of crystalline and non-crystalline materials. While several such facilities have been available around the world for some time now, few have been optimized for the handling of delicate soft-tissue specimens under cryogenic conditions. Here the development of a new X-ray micro-diffraction instrument at the Biophysics Collaborative Access Team beamline 18-ID at the Advanced Photon Source, and its use with newly developed cryo-diffraction techniques for soft-tissue studies, are described. The combination of the small beam sizes delivered by this instrument, the high delivered flux and successful cryo-freezing of rat-tail tendon has enabled us to record data to better than 4âÅ resolution. The ability to quickly raster scan samples in the beam allows selection of ordered regions in fibrous samples for markedly improved data quality. Examples of results of experiments obtainable using this instrument are presented.
File Format	HTM / HTML
ISSN	09090495
e-ISSN	16005775
DOI	10.1107/S1600577514012259
Journal	Journal of Synchrotron Radiation
Issue Number	Pt 5
Volume Number	21
Language	English
Publisher	IUCr
Publisher Date	2014-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Radiology Collagen Radiation Effects Crystallography, X-ray Instrumentation Spectrometry, X-ray Emission Synchrotrons X-ray Diffraction Alzheimer Disease Animals Equipment Design Illinois Lampreys Notochord Photons Radiography Tendons Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Nuclear and High Energy Physics Instrumentation Radiation

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X-ray micro-diffraction studies on biological samples at the BioCAT Beamline 18-ID at the Advanced Photon Source

X-ray beam stabilization at BL-17A, the protein microcrystallography beamline of the Photon Factory.

The Structural Biology Center 19ID undulator beamline: facility specifications and protein crystallographic results.

Implementation of remote monitoring and diffraction evaluation systems at the Photon Factory macromolecular crystallography beamlines.

Development of diamond-based X-ray detection for high-flux beamline diagnostics.

Performance of single-photon-counting PILATUS detector modules.

Development of a microsecond X-ray protein footprinting facility at the Advanced Light Source.

100 ps time-resolved solution scattering utilizing a wide-bandwidth X-ray beam from multilayer optics.

Improvements toward highly accurate diffraction experiments at the macromolecular micro-crystallography beamline BL-17A

X-ray micro-diffraction studies on biological samples at the BioCAT Beamline 18-ID at the Advanced Photon Source.

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X-ray micro-diffraction studies on biological samples at the BioCAT Beamline 18-ID at the Advanced Photon Source

X-ray beam stabilization at BL-17A, the protein microcrystallography beamline of the Photon Factory.

The Structural Biology Center 19ID undulator beamline: facility specifications and protein crystallographic results.

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Development of diamond-based X-ray detection for high-flux beamline diagnostics.

Performance of single-photon-counting PILATUS detector modules.

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100 ps time-resolved solution scattering utilizing a wide-bandwidth X-ray beam from multilayer optics.

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X-ray micro-diffraction studies on biological samples at the BioCAT Beamline 18-ID at the Advanced Photon Source.