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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pasilis, Sofie P. Kertesz, Vilmos Van Berkel, Gary J. Schulz, Michael Schorcht, Susanne |
| Description | Country affiliation: United States Author Affiliation: Pasilis SP ( Organic and Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA.) |
| Abstract | Desorption electrospray ionization mass spectrometry (DESI-MS) was demonstrated as a method to detect and identify peptides from two-dimensional separations of cytochrome c and myoglobin tryptic digests on ProteoChrom HPTLC Cellulose sheets. Data-dependent tandem mass spectra were acquired during lane scans across the TLC plates. Peptides and the corresponding proteins were identified using a protein database search software. Two-dimensional distributions of identified peptides were mapped for each separated protein digest. Sequence coverages for cytochrome c and myoglobin were 81 and 74%, respectively. These compared well with those determined using the more standard HPLC/ESI-MS/MS approach (89 and 84%, respectively). Preliminary results show that use of more sensitive instrumentation has the potential for improved detection of peptides with low R(f) values and improvement in sequence coverage. However, less multiple charging and more sodiation were seen in HPTLC/DESI-MS spectra relative to HPLC/ESI-MS spectra, which can affect peptide identification by MS/MS. Methods to increase multiple charging and reduce the extent of sodiation are currently under investigation. |
| File Format | HTM / HTML |
| ISSN | 10765174 |
| Issue Number | 12 |
| Volume Number | 43 |
| e-ISSN | 10969888 |
| Journal | Journal of Mass Spectrometry |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2008-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Spectroscopy Discipline Chemistry Techniques Cytochromes c Chemistry Myoglobin Peptides Analysis Amino Acid Sequence Animals Cattle Chromatography, Liquid Methods Horses Molecular Sequence Data Spectrometry, Mass, Electrospray Ionization Trypsin Metabolism Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Medicine |
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