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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mandal, Mridul Kanti Chen, Lee Chuin Yu, Zhan Nonami, Hiroshi Erra-Balsells, Rosa Hiraoka, Kenzo |
| Description | Country affiliation: Japan Author Affiliation: Mandal MK ( Clean Energy Research Center, University of Yamanashi, Kofu, Yamanashi, Japan.) |
| Abstract | Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents. |
| File Format | HTM / HTML |
| ISSN | 10765174 |
| Issue Number | 10 |
| Volume Number | 46 |
| e-ISSN | 10969888 |
| Journal | Journal of Mass Spectrometry |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2011-10-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Spectroscopy Discipline Chemistry Techniques Detergents Chemistry Proteins Spectrometry, Mass, Electrospray Ionization Instrumentation Cetrimonium Compounds Equipment Design Octoxynol Sodium Dodecyl Sulfate Solutions Methods Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Medicine |
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