NDLI: Changes in lipid distribution in E. coli strains in response to norfloxacin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Schenk, Emily R. Nau, Frederic Thompson, Christopher J. Tse-Dinh, Yuk-Ching Fernandez-Lima, Francisco
Description	Country affiliation: United States Author Affiliation: Schenk ER ( Department of Chemistry and Biochemistry, Florida International University, Miami, FL, 33199, USA.)
Abstract	Bacterial resistance to antibiotics has become an increasing threat, requiring not only the development of new targets in drug discovery, but more importantly, a better understanding of cellular response. In the current study, three closely related Escherichia coli strains, a wild type (MG1655) and an isogenic pair derived from the wild type (DPB635 and DPB636) are studied following exposure to sub lethal concentrations of antibiotic (norfloxacin) over time. In particular, genotype similarities between the three strains were assessed based on the lipid regulation response (e.g. presence/absence and up/down regulation). Lipid identification was performed using direct surface probe analysis (matrix-assisted laser desorption/ionization, MALDI), coupled to high-resolution mass spectrometry (Fourier transform ion cyclotron resonance mass spectrometry, FT-ICR MS) followed by statistical analysis of variability and reproducibility across batches using internal standards. Inspection of the lipid profile showed that for the MG1655, DPB635 and DPB636 E. coli strains, a similar distribution of the altered lipids was observed after exposure to norfloxacin antibiotic (e.g. fatty acids and glycerol phospholipids are up and down regulated, respectively). Additionally, variations in the lipid distribution resemble the extent to which each strain can combat the antibiotic exposure. That is, the topA66 topoisomerase I mutation of DPB636 translates into diminished response related to antibiotic sensitivity when compared to MG1655 and the DPB635 strains.
File Format	HTM / HTML
ISSN	10765174
e-ISSN	10969888
DOI	10.1002/jms.3500
Journal	Journal of Mass Spectrometry
Issue Number	1
Volume Number	50
Language	English
Publisher	Wiley
Publisher Date	2015-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Spectroscopy Discipline Chemistry Techniques Escherichia Coli Drug Effects Lipids Mass Spectrometry Norfloxacin Pharmacology Data Interpretation, Statistical Dose-response Relationship, Drug Chemistry Genetics Metabolism Administration & Dosage Phospholipids Reproducibility Of Results Sos Response (genetics) Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Spectroscopy, Fourier Transform Infrared Instrumentation Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Investigations with O-linked protein glycosylations by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry.

Coumarins as new matrices for matrix-assisted laser-desorption/ionization Fourier transform ion cyclotron resonance mass spectrometric analysis of hydrophobic compounds.

Ouabain-induced modifications of prostate cancer cell lipidome investigated with mass spectrometry and FTIR spectroscopy

Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories.

1,8-bis(dimethylamino)naphthalene/9-aminoacridine: a new binary matrix for lipid fingerprinting of intact bacteria by matrix assisted laser desorption ionization mass spectrometry.

Liposomes alter thermal phase behavior and composition of red blood cell membranes

Increased detection rate of melamine-containing calcium urolithiasis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique in clinical practice.

Antibacterial compounds from Zanthoxylum rhetsa

Changes in lipid distribution in E. coli strains in response to norfloxacin.

Similar Documents

Investigations with O-linked protein glycosylations by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry.

Coumarins as new matrices for matrix-assisted laser-desorption/ionization Fourier transform ion cyclotron resonance mass spectrometric analysis of hydrophobic compounds.

Ouabain-induced modifications of prostate cancer cell lipidome investigated with mass spectrometry and FTIR spectroscopy

Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories.

1,8-bis(dimethylamino)naphthalene/9-aminoacridine: a new binary matrix for lipid fingerprinting of intact bacteria by matrix assisted laser desorption ionization mass spectrometry.

Liposomes alter thermal phase behavior and composition of red blood cell membranes

Increased detection rate of melamine-containing calcium urolithiasis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique in clinical practice.

Antibacterial compounds from Zanthoxylum rhetsa

Changes in lipid distribution in E. coli strains in response to norfloxacin.