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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kuroda, Shohei Kurasawa, Masumi Mizukoshi, Koji Maeda, Tetsuo Yamamoto, Takuya Oba, Ai Kishibe, Mari Ishida-Yamamoto, Akemi |
| Description | Country affiliation: Japan Author Affiliation: Kuroda S ( Pola Chemical Industries, Inc., 560 Kashio-cho, Totsuka-ku, Yokohama 244-0812, Japan.) |
| Abstract | BACKGROUND: Polarized secretion of lamellar granules (LGs) delivers various lipids, proteases, and protease inhibitors into the stratum corneum (SC) of the epithelium. Disruption of LGs is associated with severe cutaneous diseases, but the mechanism of their polarized secretion is not known. On the other hand, recent study shows epidermal tight junctions (TJs) localize in stratum granulosum (SG), and TJs are involved in polarized molecule secretion. Thus, we hypothesized epidermal TJs relate to polarized LGs secretion. OBJECTIVE: To assess the possibility that epidermal TJs are involved in polarized LGs secretion. METHODS: In order to examine LGs secretion, we used fluorescent ceramide (BODIPY FL C(5)-ceramide) and a natural LG cargo, lympho-epithelial Kazal-type-related inhibitor (LEKTI), in cultured normal human epidermal keratinocytes and a reconstructed human epidermis. We investigated their alteration using the medium-chain fatty acid sodium caprate (C10), TJs inhibitor. In addition, LG distribution was observed by electron microscopy. RESULTS: C10 significantly inhibited secretion of both fluorescent ceramide and LEKTI in cultured normal human epidermal keratinocytes and a reconstructed human epidermis. C10 also disturbed the polarized localization of fluorescent ceramide and LEKTI in the reconstructed epidermis. Electron microscopy revealed that a large number of LGs remained in corneocytes in the C10-treated epidermis, rather than being secreted. CONCLUSION: Our data indicate that C10 perturbs the polarized secretion of LGs. Our study therefore suggests that epidermal TJs are possibly involved in polarized LG secretion and provides new insights into potential of treatments for skin diseases caused by abnormal LG secretion. |
| File Format | HTM / HTML |
| ISSN | 09231811 |
| Issue Number | 2 |
| Volume Number | 59 |
| e-ISSN | 1873569X |
| Journal | Journal of Dermatological Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Dermatology Cytoplasmic Granules Drug Effects Secretion Decanoic Acids Pharmacology Tight Junctions Physiology Cells, Cultured Ceramides Metabolism Epidermis Cytology Humans Keratinocytes Proteinase Inhibitory Proteins, Secretory Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Molecular Biology Dermatology |
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