NDLI: Monitoring of redox state in a dechlorinating culture with immobilized redox indicators

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ruiz-Haas, Peter Ingle, James
Description	Country affiliation: United States Author Affiliation: Ruiz-Haas P ( Department of Chemistry, Pearce Science Center, Mary Baldwin College, Staunton, VA 24401, USA.)
Abstract	During the dechlorination of tetrachloroethene (PCE) to ethene (ETH) in a microcosm bottle containing an enriched chlororespiring culture, redox conditions were spectrophotometrically monitored with the redox indicators thionine (THI) and cresyl violet ( CV ) immobilized on transparent films. Measurements were taken with a unique flow redox monitoring system based on circulating solution from the bottle through a specially constructed flow cell and returning it to the bottle. An enclosure for a peristaltic pump was constructed to minimize contamination of the solution with atmospheric $O_{2}.$ The redox monitoring instrumentation was shown to be an excellent tool to evaluate the ability of an enclosure for anoxic samples to prevent diffusion of very small amounts of oxygen from the atmosphere into the sample. With careful design of the apparatus, the $O_{2}$ permeation rate into a solution of THI in a microcosm bottle during redox monitoring was reduced to less than 40 nmol $h^{−1}.$ When monitoring a dechlorinating culture, dechlorination was not observed until immobilized THI was completely reduced and vinyl chloride was not produced until partial reduction of CV .
File Format	HTM / HTML
ISSN	14640325
Issue Number	5
Volume Number	11
e-ISSN	14640333
Journal	Journal of Environmental Monitoring
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2009-05-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Environmental Health Chlorine Chemistry Environmental Monitoring Methods Benzoxazines Bioreactors Diffusion Ethylenes Oxazines Oxidation-reduction Oxygen Phenothiazines Tetrachloroethylene Journal Article Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law

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