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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Gha-Young Wang, Xiaofang Son, Ahjeong |
| Description | Country affiliation: United States Author Affiliation: Kim GY ( Department of Civil Engineering, Auburn University, Auburn, AL 36849, USA.) |
| Abstract | We have demonstrated the preliminary results of the in situ monitoring capability of an inhibitor resistant gene quantification assay using magnetic bead (MB) and quantum dot (QD) nanoparticles (hereafter “MB–QD assay”) for the detection ofE. coli O157:H7 in environmental samples. The selectivity of the MB–QD assay was demonstrated via the discrimination of the target bacteria in the presence of nonspecific microbial populations. The effect of temperature on the assay was examined to evaluate the necessity of elevated temperature incubation. The reagents (i.e., particle complex and particle–DNA conjugate) were also shown to have a stability of at least 10 days without refrigeration, therefore enabling prior preparation and the subsequent storage of these reagents. In addition, it was found that the MB–QD assay was resistant to the presence of naturally occurring inhibitors (i.e., humic acids, $Ca^{2+})$ and residual reagents from DNA extraction (i.e., surfactant , ethanol ). Overall the results indicated that the MB–QD assay is potentially suitable for further development as an in situ bacteria monitoring method for working with inhibitor laden samples without requiring additional purification steps and elevated temperature processes. |
| File Format | HTM / HTML |
| ISSN | 14640325 |
| Issue Number | 5 |
| Volume Number | 13 |
| e-ISSN | 14640333 |
| Journal | Journal of Environmental Monitoring |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Publisher Date | 2011-05-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Subscribed |
| Subject Keyword | Discipline Environmental Health Anti-bacterial Agents Toxicity Drug Resistance, Bacterial Genetics Escherichia Coli O157 Quantum Dots Cations Colony Count, Microbial Methods Drug Effects Isolation & Purification Gene Expression Genes, Bacterial Humic Substances Metals Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law |
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