NDLI: Agmatine attenuates silica-induced pulmonary fibrosis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	El-Agamy, D. S. Sharawy, M. H. Ammar, E. M.
Description	Country affiliation: Egypt Author Affiliation: El-Agamy DS ( Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt dinaagamy1@yahoo.com.); Sharawy MH ( Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.); Ammar EM ( Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.)
Abstract	There is a large body of evidence that nitric oxide (NO) formation is implicated in mediating silica-induced pulmonary fibrosis. As a reactive free radical, NO may not only contribute to lung parenchymal tissue injury but also has the ability to combine with superoxide and form a highly reactive toxic species peroxynitrite that can induce extensive cellular toxicity in the lung tissues. This study aimed to explore the effect of agmatine, a known NO synthase inhibitor, on silica-induced pulmonary fibrosis in rats. Male Sprague Dawley rats were treated with agmatine for 60 days following a single intranasal instillation of silica suspension (50 mg in 0.1 ml saline/rat). The results revealed that agmatine attenuated silica-induced lung inflammation as it decreased the lung wet/dry weight ratio, protein concentration, and the accumulation of the inflammatory cells in the bronchoalveolar lavage fluid. Agmatine showed antifibrotic activity as it decreased total hydroxyproline content of the lung and reduced silica-mediated lung inflammation and fibrosis in lung histopathological specimen. In addition, agmatine significantly increased superoxide dismutase (p < 0.001) and reduced glutathione (p < 0.05) activities with significant decrease in the lung malondialdehyde (p < 0.001) content as compared to the silica group. Agmatine also reduced silica-induced overproduction of pulmonary nitrite/nitrate as well as tumor necrosis factor . Collectively, these results demonstrate the protective effects of agmatine against the silica-induced lung fibrosis that may be attributed to its ability to counteract the NO production, lipid peroxidation, and regulate cytokine effects.
File Format	HTM / HTML
ISSN	09603271
Issue Number	6
Volume Number	33
e-ISSN	14770903
Journal	Human & Experimental Toxicology
Language	English
Publisher	Sage Publication
Publisher Date	2014-06-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Toxicology Agmatine Pharmacology Enzyme Inhibitors Lung Drug Effects Nitric Oxide Synthase Antagonists & Inhibitors Nitric Oxide Metabolism Pulmonary Fibrosis Prevention & Control Silicon Dioxide Animals Biological Markers Bronchoalveolar Lavage Fluid Chemistry Cytology Cytoprotection Disease Models, Animal Glutathione Hydroxyproline Lipid Peroxidation Enzymology Pathology Male Malondialdehyde Nitrates Nitrites Pneumonia Chemically Induced Pulmonary Edema Rats, Sprague-dawley Superoxide Dismutase Time Factors Journal Article
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology

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