NDLI: High-throughput DNA extraction of forensic adhesive tapes.

Please wait, while we are loading the content...

Corrigendum to 'Evaluation of the IrisPlex DNA-based eye color prediction assay in a United States population' [Forensic Sci. Int. Genet. (2014) 111-117].

Comparison of DNA polymerases for improved forensic analysis of challenging samples.

Frequencies of HID-ion ampliseq ancestry panel markers among greenlanders.

Development of a methylation marker set for forensic age estimation using analysis of public methylation data and the Agena Bioscience EpiTYPER system.

Is technology the death of expertise?

DNA methylation profiling for a confirmatory test for blood, saliva, semen, vaginal fluid and menstrual blood.

Next generation sequencing: Improved resolution for paternal/maternal duos analysis.

Massively parallel sequencing of short tandem repeats-Population data and mixture analysis results for the PowerSeq™ system.

Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal Short Tandem Repeat allele frequency databasing (STRidER).

Big bad wolf or man's best friend? Unmasking a false wolf aggression on humans.

Concordance and reproducibility of a next generation mtGenome sequencing method for high-quality samples using the Illumina MiSeq.

Population data of 15 autosomal STR loci in Chinese Han population from Jiangsu Province, Eastern China.

A novel cell culture model as a tool for forensic biology experiments and validations.

Forensic Spanish allele and haplotype database for a 17 X-STR panel.

Probabilistically determining the cellular source of DNA derived from differential extractions in sexual assault scenarios.

The mitochondrial DNA makeup of Romanians: A forensic mtDNA control region database and phylogenetic characterization.

Adventitious match probability for autosomal profiles when primer binding site mutation is possible.

Native American population data based on the Globalfiler(®) autosomal STR loci.

Genetic polymorphism of 22 autosomal STR markers in a Han population of Southern China.

Genetic polymorphic investigation of 21 autosomal short tandem repeat loci in the Chinese Li ethnic group.

Shedding light on the relative DNA contribution of two persons handling the same object.

A general review of the most common COI primers for Calliphoridae identification in forensic entomology.

High-throughput DNA extraction of forensic adhesive tapes.

Developmental validation of the Yfiler(®) Plus PCR Amplification Kit: An enhanced Y-STR multiplex for casework and database applications.

Off-ladder alleles due to a single nucleotide polymorphism in the flanking region at DYS481 detected by the PowerPlex(®) Y23 System.

Biohistorical materials and contemporary privacy concerns-the forensic case of King Albert I.

Differences in urbanization degree and consequences on the diversity of conventional vs. rapidly mutating Y-STRs in five municipalities from a small region of the Tyrolean Alps in Austria.

Mitochondrial DNA control region haplotype and haplogroup diversity in South Eastern Turkey.

GlobalFiler(®) Express DNA amplification kit in South Africa: Extracting the past from the present.

Year: 2016 Volume: 23

Year: 2016 Volume: 22

Year: 2016 Volume: 21

Year: 2016 Volume: 20

Year: 2015 Volume: 19

Year: 2015 Volume: 18

Year: 2015 Volume: 17

Year: 2015 Volume: 16

Year: 2015 Volume: 15

Year: 2015 Volume: 14

Year: 2014 Volume: 13

Year: 2014 Volume: 12

Year: 2014 Volume: 11

Year: 2014 Volume: 10

High-throughput DNA extraction of forensic adhesive tapes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Forsberg, Christina Jansson, Linda Ansell, Ricky Hedman, Johannes
Description	Country affiliation: Sweden Author Affiliation: Forsberg C ( Swedish National Forensic Centre, Linköping, Sweden.); Jansson L ( Applied Microbiology, Department of Chemistry, Lund University, Lund, Sweden.); Ansell R ( Swedish National Forensic Centre, Linköping, Sweden); Hedman J ( Swedish National Forensic Centre, Linköping, Sweden)
Abstract	Tape-lifting has since its introduction in the early 2000's become a well-established sampling method in forensic DNA analysis. Sampling is quick and straightforward while the following DNA extraction is more challenging due to the 'stickiness', rigidity and size of the tape. We have developed, validated and implemented a simple and efficient direct lysis DNA extraction protocol for adhesive tapes that requires limited manual labour. The method uses Chelex beads and is applied with SceneSafe FAST tape. This direct lysis protocol provided higher mean DNA yields than PrepFiler Express BTA on Automate Express, although the differences were not significant when using clothes worn in a controlled fashion as reference material (p=0.13 and p=0.34 for T-shirts and button-down shirts, respectively). Through in-house validation we show that the method is fit-for-purpose for application in casework, as it provides high DNA yields and amplifiability, as well as good reproducibility and DNA extract stability. After implementation in casework, the proportion of extracts with DNA concentrations above 0.01ng/µL increased from 71% to 76%. Apart from providing higher DNA yields compared with the previous method, the introduction of the developed direct lysis protocol also reduced the amount of manual labour by half and doubled the potential throughput for tapes at the laboratory. Generally, simplified manual protocols can serve as a cost-effective alternative to sophisticated automation solutions when the aim is to enable high-throughput DNA extraction of complex crime scene samples.
File Format	HTM / HTML
ISSN	18724973
Journal	Forensic Science International: Genetics
Volume Number	24
e-ISSN	18780326
Language	English
Publisher	Elsevier
Publisher Date	2016-09-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Forensic Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Development of a normalized extraction to further aid in fast, high-throughput processing of forensic DNA reference samples.

Chemical fingerprinting of adhesive tapes by GCMS detection of petroleum hydrocarbon products.

Classification of OPP adhesive tapes according to pyrogram of adhesives.

High-throughput Sequencing of Trace Quantities of Soil Provides Reproducible and Discriminative Fungal DNA Profiles.

Classification of OPP adhesive tapes according to MALDI mass spectra of adhesives.

Less is More--Optimization of DNA Extraction from Canine Feces.

Sequence discrimination of heterogeneous crossing of seal impression and ink-printed text using adhesive tapes.

Analysis and discrimination of electrical tapes: part II. Backings.

Analysis and discrimination of electrical tapes: Part I. Adhesives.

High-throughput DNA extraction of forensic adhesive tapes.

Similar Documents

Development of a normalized extraction to further aid in fast, high-throughput processing of forensic DNA reference samples.

Chemical fingerprinting of adhesive tapes by GCMS detection of petroleum hydrocarbon products.

Classification of OPP adhesive tapes according to pyrogram of adhesives.

High-throughput Sequencing of Trace Quantities of Soil Provides Reproducible and Discriminative Fungal DNA Profiles.

Classification of OPP adhesive tapes according to MALDI mass spectra of adhesives.

Less is More--Optimization of DNA Extraction from Canine Feces.

Sequence discrimination of heterogeneous crossing of seal impression and ink-printed text using adhesive tapes.

Analysis and discrimination of electrical tapes: part II. Backings.

Analysis and discrimination of electrical tapes: Part I. Adhesives.

High-throughput DNA extraction of forensic adhesive tapes.