NDLI: Efficacy of chemical treatments against murine norovirus, feline calicivirus, and MS2 bacteriophage.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	D'Souza, Doris H. Su, Xiaowei
Description	Country affiliation: United States Author Affiliation: D'Souza DH ( Department of Food Science and Technology, The University of Tennessee, Knoxville, Tennessee 37996-4591, USA.)
Abstract	Human noroviruses pose an emerging public health threat, and despite stringent control strategies, variant strains continue to spread and cause disease outbreaks. Routinely used chemical sanitizers, such as sodium hypochlorite though effective on food contact surfaces, require high concentrations to cause reduction in enteric viral titers. The aim of this study was to evaluate the efficacy of trisodium phosphate (TSP) against three human enteric virus surrogates, murine norovirus (MNV-1), feline calicivirus (FCV), and bacteriophage MS2 in comparison to routinely used sanitizers. Three concentrations of TSP (1%, 2%, and 5%) at different contact times (30 sec and 1 min) were evaluated against the surrogate viruses individually inoculated on formica coupons. Our results showed that 5% TSP was effective in obtaining a >or=6 log(10) PFU reduction for MNV-1, FCV, and MS2 after a contact time of only 30 sec or 1 min similar to 10% household bleach (0.6% sodium hypochlorite, 5000 ppm available chlorine) for high titers of FCV and MS2, and with approximately 5 log(10) reduction after either 30 sec or 1 min on low viral titers. However, 2% TSP for 1 min resulted in >or=6 log(10) PFU reduction for FCV and MS2, but only a 1.05 log(10) PFU reduction for MNV-1 at high titers, with similar results after 30 sec. Decreasing TSP levels to 1% reduced FCV by approximately 2.65 log(10) PFU, MS2 by 4.5 log(10) PFU at high titers, and no reduction for MNV-1 after 30-sec or 1 min contact. Glutaraldehyde (2%) reduced FCV and MNV-1 titers by approximately 6 log(10) PFU; however, MS2 was reduced by only 3.22 and 3.74 log(10) PFU after 30 sec and 1 min, respectively, while 70% ethanol was not effective in reducing the three viruses at either high or low titers at both contact times. Alternative control strategies using TSP with short contact times should benefit the food industry in reducing norovirus transmission.
File Format	HTM / HTML
ISSN	15353141
Issue Number	3
Volume Number	7
e-ISSN	15567125
Journal	Foodborne Pathogens and Disease
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-03-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Microbiology Discipline Parasitology Discipline Nutritional Sciences Antiviral Agents Pharmacology Caliciviridae Infections Prevention & Control Levivirus Drug Effects Norovirus Animals Administration & Dosage Transmission Cats Disinfectants Ethanol Food Handling Instrumentation Foodborne Diseases Virology Gastroenteritis Glutaral Humans Mice Phosphates Sodium Hypochlorite Solutions Viral Load Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Microbiology Applied Microbiology and Biotechnology Food Science

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