NDLI: Genetic Characterization of Extended-Spectrum Beta-Lactamases in Escherichia coli Isolates of Pigs from a Portuguese Intensive Swine Farm

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gonçalves, Alexandre Torres, Carmen Silva, Nuno Carneiro, Catarina Radhouani, Hajer Coelho, Céline Araújo, Carlos Rodrigues, Jorge Vinué, Laura Somalo, Sergio Poeta, Patrícia Igrejas, Gilberto
Spatial Coverage	Portugal
Description	Country affiliation: Portugal Author Affiliation: Gonçalves A ( Center of Genetics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.)
Abstract	There is a great concern by the emergence and the wide dissemination of extended-spectrum beta-lactamases (ESBLs) among animal Escherichia coli isolates. We intended to determinate the carriage level and type of ESBLs in E. coli obtained from fecal samples from pigs raised on an intensive pig farm in Portugal; further to characterize other associated resistance genes and their plasmid content, the phylogenetic groups, and the clonal relationship of ESBL-positive isolates. Sixty-five fecal samples were seeded in Levine media supplemented with cefotaxime for E. coli recovery. Susceptibility to 16 antimicrobial agents was performed by disk diffusion agar. ESBL-phenotypic detection was carried out by double-disk test; and the presence of the genes encoding TEM, OXA, SHV, and CTX-M type beta-lactamases was studied by polymerase chain reaction and sequencing. Other mechanisms of antimicrobial resistance and phylogenetic groups were also determined. Clonal relationship was performed by pulsed-field gel electrophoresis. ESBL-producing E. coli isolates were detected in 16 fecal samples, and one isolate per sample was studied. The CTX-M-1 type ESBL was detected in the 16 isolates. The gene encoding TEM-1 was identified to be associated with eight CTX-M-1-positive isolates. The tet(A) gene was found in 12 of 14 tetracycline-resistant isolates, and the aadA or strA-strB genes were found in the streptomycin-resistant isolates. Fourteen and two ESBL-containing isolates belonged to A and B1 phylogenetic groups, respectively. Clonal relationship of ESBL-containing isolates identified seven unrelated patterns. Swine represent an important reservoir of ESBL-containing E. coli isolates, especially of the CTX-M-1 type.
File Format	HTM / HTML
ISSN	15353141
Issue Number	12
Volume Number	7
e-ISSN	15567125
Journal	Foodborne Pathogens and Disease
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-12-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Microbiology Discipline Parasitology Discipline Nutritional Sciences Drug Resistance, Bacterial Escherichia Coli Proteins Genetics Escherichia Coli Enzymology Swine Microbiology Beta-lactamases Animals Electrophoresis, Gel, Pulsed-field Drug Effects Growth & Development Isolation & Purification Feces Genes, Mdr Genetic Markers Microbial Sensitivity Tests Plasmids Polymerase Chain Reaction Portugal Journal Article
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Microbiology Applied Microbiology and Biotechnology Food Science

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