NDLI: Correlation of fluoroquinolone resistance with expression of qnrA gene in Kluyvera spp.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Huang, Si-Yang Zhu, Xing-Quan Wang, Yang Dai, Lei Wang, Zhan-Hui Li, Bei-Bei Wu, Cong-Ming Shen, Jian-Zhong
Spatial Coverage	China
Description	Author Affiliation: Huang SY ( State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, The People's Republic of China.)
Abstract	The objective of the present study was to examine whether the expression of qnrA may contribute to a high level of resistance among parent and induced strains of Kluyvera spp. Two clinical isolates of ciprofloxacin-resistant Kluyvera spp. were obtained from livers of diseased chickens, and upon induction with ciprofloxacin, six strains with increased resistance were produced. Point mutations in qnrA, aac(6')-Ib-cr, gyrA, gyrB, parC, and parE were investigated by polymerase chain reaction (PCR) amplification and DNA sequencing, and expression levels of acrAB and qnrA in all strains were investigated by quantitative real-time PCR (qRT-PCR). The induced strains contained the same mutations in quinolone resistance-determining region as those of the parent strains. qRT-PCR showed that the expression of the acrA gene was not detected in any strain and acrB gene expression was unchanged between induced and parental strains. However, difference in expression of qnrA was observed, which correlated well with the level of quinolone resistance in the parent and induced strains. The induced high resistance was not affected by mutations in qnrA and aac(6')-Ib-cr, by new mutations in the quinolone resistance-determining region of gyrA, gyrB, parC, and parE, or by the expression level of acrAB. These data suggest that the expression of qnrA may be a factor contributing to the high level of resistance among parent and induced strains of Kluyvera spp.
File Format	HTM / HTML
ISSN	15353141
Issue Number	9
Volume Number	8
e-ISSN	15567125
Journal	Foodborne Pathogens and Disease
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2011-09-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Microbiology Discipline Parasitology Discipline Nutritional Sciences Anti-bacterial Agents Pharmacology Bacterial Proteins Metabolism Drug Resistance, Multiple, Bacterial Fluoroquinolones Gene Expression Regulation, Bacterial Drug Effects Kluyvera Animals Genetics Chickens Microbiology China Dna Mutational Analysis Foodborne Diseases Drug Therapy Isolation & Purification Liver Microbial Sensitivity Tests Mutant Proteins Osmolar Concentration Point Mutation Poultry Diseases Quinolones Real-time Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Microbiology Applied Microbiology and Biotechnology Food Science

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