NDLI: Cytokine and catabolic enzyme expression in synovium, synovial fluid and articular cartilage of naturally osteoarthritic equine carpi.

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Cytokine and catabolic enzyme expression in synovium, synovial fluid and articular cartilage of naturally osteoarthritic equine carpi.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kamm, J. L. Nixon, A. J. Witte, T. H.
Description	Country affiliation: United States Author Affiliation: Kamm JL ( College of Veterinary Medicine, Cornell University, NY, USA.)
Abstract	REASONS FOR PERFORMING STUDY: Understanding the expression of catabolic and anabolic genes during osteoarthritis progression should help to identify the major mediators of the disease. OBJECTIVE: To compare the cytokine and anabolic marker concentrations in synovium, synovial fluid and cartilage between normal and osteoarthritic joints. METHODS: Carpi from horses age 2-11 years were used. Tissues were harvested at the time of surgery or euthanasia, and RNA was isolated for RT-PCR analysis. Tumour necrosis factor alpha (TNF ), interleukin-1beta (IL-1ß), aggrecanase 1 (ADAMTS-4), aggrecanase 2 (ADAMTS-5), matrix metalloproteinase-13 (MMP-13), interleukin 17 (IL-17) and collagen type I alpha 1(Col-1) expression were determined in synovium. TNF , IL-1ß, ADAMTS-4, ADAMTS-5, MMP-13, IL-17, collagen type IIB (Col-2B), and aggrecan expression were determined in cartilage. TNF concentration in the synovial fluid was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Expression of TNF , ADAMTS-5 and MMP-13 was significantly increased in synovial tissue from OA joints. Synovial membrane IL-1ß abundance showed only moderate elevations in OA, without reaching significant levels. Cytokine expression was increased significantly in OA cartilage samples, particularly TNF , IL-1ß, ADAMTS-4 and MMP-13; and collagen type I expression was significantly increased in synovial tissues from OA groups. Collagen type II message was diminished in mild and moderate stages of OA, but rebounded to significant elevations in severely degenerate joints. Conversely, aggrecan levels significantly declined in cartilage from all OA groups. Synovial fluid TNF peptide concentration was significantly increased in severe OA cases. CONCLUSION: TNF was increased in all degrees of equine OA, and was abundantly expressed in synovial membrane and cartilage. IL-1ß was overexpressed in OA cartilage, but not to a significant extent in synovium. POTENTIAL RELEVANCE: Control of TNF should be considered further as a target in the treatment of OA. ADAMTS-4 may be the primary aggrecanase causing cartilage breakdown in OA.
File Format	HTM / HTML
ISSN	04251644
e-ISSN	20423306
DOI	10.1111/j.2042-3306.2010.00140.x
Journal	Equine Veterinary Journal
Issue Number	8
Volume Number	42
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2010-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Veterinary Medicine Cartilage, Articular Metabolism Cytokines Gene Expression Regulation Physiology Horse Diseases Osteoarthritis Veterinary Synovial Membrane Animals Genetics Endopeptidases Horses Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Equine

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Cytokine and catabolic enzyme expression in synovium, synovial fluid and articular cartilage of naturally osteoarthritic equine carpi

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Cytokine and catabolic enzyme expression in synovium, synovial fluid and articular cartilage of naturally osteoarthritic equine carpi.

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