NDLI: Pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad Alytes obstetricans on the Iberian Peninsula.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Balseiro, Ana Dalton, Kevin P. Del Cerro, Ana Marquez, Isabel Cunningham, Andrew A. Parra, Francisco Prieto, José M. Casais, R.
Spatial Coverage	Spain
Description	Country affiliation: Spain Author Affiliation: Balseiro A ( SERIDA, Servicio Regional de Investigación y Desarrollo Agroalimentario, Laboratorio de Sanidad Animal, 33299 Jove, Gijón, Spain. abalseiro@serida.org)
Abstract	We describe the pathology, isolation and characterisation of a virus responsible for an outbreak of a systemic haemorrhagic disease causing high mortality in tadpoles of the common midwife toad Alytes obstetricans in the 'Picos de Europa' National Park in northern Spain. The virus, provisionally designated as the common midwife toad virus (CMTV), was isolated from homogenates of visceral tissue from diseased toad tadpoles following inoculation on epithelioma papulosum cyprini (EPC) cells. Molecular characterisation of the virus, including sequence analysis of the DNA polymerase and major capsid protein genes, showed that the isolated virus was a ranavirus with marked sequence identity to other members of the genus Ranavirus. A rabbit antiserum raised against purified virions was prepared and used to definitively demonstrate systemic distribution of the virus in diseased tadpoles, indicating that the isolated virus was the primary pathogen.
File Format	HTM / HTML
ISSN	01775103
e-ISSN	16161580
Journal	Diseases of Aquatic Organisms
Issue Number	2
Volume Number	84
Language	English
Publisher	Federal Republic of Germany : Inter-Research
Publisher Date	2009-04-06
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Discipline Veterinary Medicine Discipline Biology Bufonidae Virology Ranavirus Classification Isolation & Purification Amino Acid Sequence Animals Dna Virus Infections Pathology Veterinary Larva Molecular Sequence Data Phylogeny Spain Viral Proteins Chemistry Genetics
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Ecology, Evolution, Behavior and Systematics

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