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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Glasier, Mary-Ann Keech, Adam Sheardown, Heather Subbaraman, Lakshman N. Jones, Lyndon |
| Description | Country affiliation: Canada Author Affiliation: Glasier MA ( Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.) |
| Abstract | PURPOSE: To compare two solvents for retrieval of lysozyme deposited on a silicone hydrogel (SH) contact lens material galyfilcon A (GA; Acuvue Advance). METHODS: Two buffers used were 50:50 acetonitrile/0.02% trifluoroacetic acid (buffer 1) and 50:50 acetonitrile/50 mM NaOH (buffer 2). RESULTS: Extraction efficiency from GA lenses was 74% (buffer 1) and 83% (buffer 2). Buffer 2 decreased lysozyme activity > buffer 1. Ex vivo GA lenses showed total protein deposition of 2-16 microg/lens with total lysozyme deposition of 0.3-3.9 microg/lens. CONCLUSIONS: We have developed a low acid strength extraction buffer that can be used to efficiently extract active lysozyme protein from novel siloxane-based contact lens materials. |
| File Format | HTM / HTML |
| ISSN | 02713683 |
| Issue Number | 1 |
| Volume Number | 33 |
| e-ISSN | 14602202 |
| Journal | Current Eye Research |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2008-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Ophthalmology Contact Lenses, Hydrophilic Hydrogels Muramidase Isolation & Purification Silicones Adsorption Blotting, Western Buffers Humans Analysis Solvents Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ophthalmology Sensory Systems Cellular and Molecular Neuroscience |
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