NDLI: Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro

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Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro

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Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Ling-Yun Ou, Min Huang, You-Zhang Qiao, Yuan-Yuan Zhang, Da-Jin
Description	Country affiliation: China Author Affiliation: Zhang LY ( Institute of Very Important Person Pulmonary Medicine, Navy General Hospital of People's Liberation Army, Beijing 100048, China.)
Abstract	Methods Twenty male Sprague-Dawley (SD) rats were randomly: divided into four groups: saline, and 0.66, 3.30 and 16.50 g/kg of YFHXG groups, the saline and different concentrations of YFHXG were given twice daily for 7 days, respectively. Serum-pharmacology method was used in the preparation of YFHXG serum. Tissue block anchorage was employed in the primary culture of rat PASMCs. The PASMCs were randomly divided into normoxia group, hypoxia group, and hypoxia+YFHXG group (0.66, 3.30 and 16.50 g/kg doses of YFHXG-treated serum groups, exposed to hypoxic condition). PASMCs in normoxia and hypoxia group were cultured with saline serum, hypoxia+YFHXG groups were cultured with different concentrations of YFHXG serum. Cell viability was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle was analyzed using flow cytometry. In addition, hypoxia inducible factor-1-alpha (HIF-1α) protein expression was evaluated by immunocytochemistry analysis, the concentration of intracellular reactive oxygen species (ROS) and Ca $Ca^{2+}$ were determined by laser scanning confocal microscopy (LSCM).
File Format	HTM / HTML
ISSN	16720415
Issue Number	7
Volume Number	18
e-ISSN	19930402
Journal	Chinese Journal of Integrative Medicine
Language	English
Publisher	Springer
Publisher Date	2012-07-01
Publisher Place	China
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Therapeutics Drugs, Chinese Herbal Pharmacology Myocytes, Smooth Muscle Cytology Drug Effects Pulmonary Artery Animals Calcium Metabolism Cell Cycle Cell Hypoxia Cell Proliferation Cell Survival Hypoxia-inducible Factor 1, Alpha Subunit Intracellular Space Male Rats Rats, Sprague-dawley Reactive Oxygen Species Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Pharmacology (medical) Complementary and Alternative Medicine

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