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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dang, Xiao-Yan Dong, Lei Shi, Hai-Tao Zou, Bai-Cang |
| Description | Country affiliation: China Author Affiliation: Dang XY ( Department of Gastroenterology, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.) |
| Abstract | OBJECTIVE: To investigate the effects of serum containing Chinese medicine (CM) Sanpi Pingwei (, SPPW) formula on the proliferation and apoptosis of human SGC-7901 cells and the possible mechanism. METHODS: Serum containing CM SPPW formula (SPPW serum) was prepared by a serum pharmacology method. Human SGC-7901 cells were incubated with SPPW serum at three different concentrations and with the anticancer drug 5-fluorouracil (5-FU), respectively. Cell proliferation was assessed by MTT assay, and cell apoptosis was detected by flow cytometry assay. Real-time quantitative polymerase chain reaction (RT-PCR) and Western blot assay were employed to confirm the expressions of Bcl-2, Bax and p53 in SGC-7901 cells at mRNA and protein levels, respectively. RESULTS: SPPW serum suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner. The colony forming rate of negative control was 48.2%, while those in the three SPPW serum groups and the 5-FU group decreased significantly (P<0.01). The number of colony forming units in the SPPW high dosage group was significantly smaller than that in the 5-FU group (P<0.01). MTT assay showed that SPPW serum restrained the proliferation of SGC-7901 cells, and the inhibition rate increased significantly in a dose-dependent manner. Annexin V/PI Assay suggested that SPPW serum induced the apoptosis of SGC-7901 cells significantly. RT-PCR and western blot assay indicated that SPPW serum upregulated the protein and mRNA expression levels of Bax and p53 in SGC-7901 cells, but downregulated the protein and mRNA expressions of Bcl-2. CONCLUSIONS: SPPW formula inhibits the proliferation of SGC-7901 cells in vitro and induces the cell apoptosis. It plays an anticancer role by regulating the expressions of Bax, p53 and Bcl-2 in SGC-7901 cells. |
| File Format | HTM / HTML |
| ISSN | 16720415 |
| Issue Number | 2 |
| Volume Number | 19 |
| e-ISSN | 19930402 |
| Journal | Chinese Journal of Integrative Medicine |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2013-02-01 |
| Publisher Place | China |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Therapeutics Apoptosis Drug Effects Drugs, Chinese Herbal Pharmacology Serum Chemistry Animals Blotting, Western Cell Line, Tumor Cell Proliferation Fluorouracil Gene Expression Regulation, Neoplastic Humans Rna, Messenger Genetics Metabolism Rats Rats, Sprague-dawley Tumor Stem Cell Assay Tumor Suppressor Protein P53 Bcl-2-associated X Protein Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Complementary and Alternative Medicine |
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