NDLI: The detection of chromosomal aneusomy by fluorescence in situ hybridization in sputum predicts lung cancer incidence.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Varella-Garcia, Marileila Schulte, Aline P. Wolf, Holly J. Feser, William J. Zeng, Chan Braudrick, Sarah Yin, Xiang Hirsch, Fred R. Kennedy, Timothy C. Keith, Robert L. Barón, Anna E. Belinsky, Steven A. Miller, York E. Byers, Tim Franklin, Wilbur A.
Description	Country affiliation: United States Author Affiliation: Varella-Garcia M ( University of Colorado Denver, 12801 East 17th Avenue, Aurora, CO 80045, USA. Marileila.Garcia@ucdenver.edu)
Abstract	Lung cancer usually is disseminated (advanced) and has a poor prognosis at diagnosis. Current and former smokers are at a high risk for lung cancer and are candidates for prevention and early detection strategies. Sputum is a potential source of biomarkers that might determine either lung cancer risk or the presence of early lung cancer, but no current sputum test is sufficiently sensitive and specific for effective screening. We used fluorescence in situ hybridization (FISH) to measure chromosomal aneusomy (CA) in sputum samples collected prospectively from 100 incident lung cancer cases and 96 controls (matched on age, gender, and date of collection) nested within an ongoing high-risk cohort. The CA-FISH assay was aimed at four DNA targets: epidermal growth factor receptor, MYC, 5p15, and CEP 6. The sensitivity of a positive CA-FISH assay (abnormal for two or more of the four markers) for lung cancer was substantially higher for samples collected within 18 months (76% sensitivity) than for samples collected more than 18 months (31%) before lung cancer diagnosis. Sensitivity was higher for squamous cell cancers (94%) than for other histologic types (69%). CA-FISH specificity based on samples collected within 18 months before diagnosis was 88%. The adjusted odds ratio (OR) of lung cancer for specimens collected within 18 months before a cancer diagnosis was higher for the CA-FISH assay [OR, 29.9; 95% confidence interval (95% CI), 9.5-94.1] than for previously studied ORs of cytologic atypia (OR, 1.8; 95% CI, 1.3-2.6) and gene promoter methylation (OR, 6.5; 95% CI, 1.2-35.5). Whether CA-FISH is an indicator of extreme risk for incident lung cancer or detects exfoliated cancer cells is unknown. The apparent promise of CA-FISH in sputum for assessing lung cancer risk and/or for lung cancer early detection now needs to be validated in a clinical screening trial.
File Format	HTM / HTML
ISSN	19406207
e-ISSN	19406215
DOI	10.1158/1940-6207.CAPR-09-0165
Journal	Cancer Prevention Research
Issue Number	4
Volume Number	3
Language	English
Publisher	American Association for Cancer Research
Publisher Date	2010-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Oncology Tumor Markers, Biological Genetics In Situ Hybridization, Fluorescence Lung Neoplasms Diagnosis Epidemiology Sputum Cytology Area Under Curve Neoplasm Staging Roc Curve Sensitivity And Specificity Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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