NDLI: Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Visniauskas, Bruna Oliveira, Vitor Carmona, Adriana K. D'Almeida, Vânia de Melo, Robson L. Tufik, Sérgio Chagas, Jair R.
Description	Country affiliation: Brazil Author Affiliation: Visniauskas B ( Departamento de Psicobiologia, Universidade Federal de São Paulo (UNIFESP), Rua Napole)
Abstract	Proteases are essential either for the release of neuropeptides from active or inactive proteins or for their inactivation. Neuropeptides have a fundamental role in sleep-wake cycle regulation and their actions are also likely to be regulated by proteolytic processing. Using fluorescence resonance energy transfer substrates, specific protease inhibitors and real-time PCR we demonstrate changes in angiotensin I-converting enzyme (ACE) expression and proteolytic activity in the central nervous system in an animal model of paradoxical sleep deprivation during 96 h (PSD). Male rats were distributed into five groups (PSD, 24 h, 48 h and 96 h of sleep recovery after PSD and control). ACE activity and mRNA levels were measured in hypothalamus, hippocampus, brainstem, cerebral cortex and striatum tissue extracts. In the hypothalamus, the significant decrease in activity and mRNA levels, after PSD, was only totally reversed after 96 h of sleep recovery. In the brainstem and hippocampus, although significant, changes in mRNA do not parallel changes in ACE specific activity. Changes in ACE activity could affect angiotensin II generation, angiotensin 1-7, bradykinin and opioid peptides metabolism. ACE expression and activity modifications are likely related to some of the physiological changes (cardiovascular, stress, cognition, metabolism function, water and energy balance) observed during and after sleep deprivation.
File Format	HTM / HTML
ISSN	14316730
Issue Number	6
Volume Number	392
e-ISSN	14374315
Journal	Biological Chemistry
Language	English
Publisher	Walter de Gruyter
Publisher Date	2011-04-01
Publisher Place	Germany
Access Restriction	Subscribed
Subject Keyword	Discipline Biochemistry Central Nervous System Enzymology Peptidyl-dipeptidase A Genetics Metabolism Sleep Deprivation Animals Enzyme Activation Gene Expression Profiling Gene Expression Regulation, Enzymologic Male Rna, Messenger Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in