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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bashir, Shanaz Singh, Rashmi Sharma, Barkha Yadav, Sharad K. |
| Description | Country affiliation: India Author Affiliation: Bashir S ( Department of Epidemiology and Vety. Preventive medicine, College of Veterinary Sciences, Veterinary University, Mathura (UP), India.) |
| Abstract | OBJECTIVE: To develop a standard enzyme-linked immunosorbent assay (ELISA) for the detection of bovine herpesvirus type 1 (BHV-1). METHODS: The assay was based on hyperimmune rabbit and guinea pig antisera raised against purified BHV-1. Polyethylene glycol precipitation and sucrose density gradient methods were adopted for viral concentration and purification. Antisera were raised using Freund's adjuvant followed by extraction of IgG of high purity. RESULTS: Optimum antisera dilutions as determined by titrations were chosen as 1:4 000, whereas the conjugate was used at 1:2 000 dilution. Using 95 clinical specimens, the ELISA test showed a sensitivity and specificity of 91.90 % and 93.10 %, respectively when compared to PCR. The cut-off value was fixed at 0.15 (A(490)) and a P/N ratio of >1.30 indicated a significant positive reaction. CONCLUSIONS: The results have demonstrated that this ELISA could efficiently detect BHV-1 and can be used as an important diagnostic tool. |
| File Format | HTM / HTML |
| ISSN | 19957645 |
| e-ISSN | 23524146 |
| Journal | Asian Pacific Journal of Tropical Medicine |
| Issue Number | 5 |
| Volume Number | 4 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-05-01 |
| Publisher Place | China |
| Access Restriction | Open |
| Subject Keyword | Discipline Tropical Medicine Enzyme-linked Immunosorbent Assay Herpesvirus 1, Bovine Isolation & Purification Animals Cells, Cultured Guinea Pigs Rabbits Sensitivity And Specificity Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
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