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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Guo, Xiu-Li Wang, Ya-Jie Cui, Pei-Lin Wang, Yan-Bin Liang, Pi-Xia Zhang, Ya-Nan Xu, You-Qing |
| Description | Country affiliation: China Author Affiliation: Guo XL ( Department of Digestive System, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.); Wang YJ ( Central Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.); Cui PL ( Department of Digestive System, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.); Wang YB ( Department of Digestive System, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.); Liang PX ( Department of Digestive System, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.); Zhang YN ( Department of Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.); Xu YQ ( Department of Digestive System, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China. Electronic address: youqingxuu@hotmail.com.) |
| Abstract | OBJECTIVE: To discuss the effect of BRMS1 on the proliferation, migration and adhesion of mouse forestomach carcinoma. METHODS: The constructed pCMV-myc-BRMS1 recombinant plasmid and blank plasmid were transfected into mouse forestomach carcinoma. MTT method was employed to measure the activity of gastric cancer cell; the scratch assay and Transwell assay to measure the migration and invasion of gastric cancer cell; the adhesion assay to measure the adhesion of gastric cancer cell; while the Western blot assay to measure the expression of The NF-κB signal pathway, downstream matrix metalloproteinase (MMP)-2, MMP-9 and osteopontin and E-cadherin in the gastric cancer cell. Besides, the transplanted animal model of gastric cancer in mice was constructed to measure the size of tumor xenograft. RESULTS: Results of MTT assay showed that, compared with the empty vector control group, the activity of gastric cancer cell was not affected in the BRMS1 transfection group. The improved expression of BRMS1 could inhibit the adhesion, migration and invasion of gastric cancer cell (P < 0.01). Besides, compared with the empty vector control group, the phosphorylation of NF-κB p65 and IκB was reduced in the BRMS1 transfection group, with the decreased expression of MMP-2, MMP-9 and osteopontin and the increased expression of E-cadherin (P < 0.01). Results of animal experiment also showed that the expression of BRMS1 did not affect the transplanted tumor. CONCLUSIONS: The expression of BRMS1 can significantly inhibit the adhesion, migration, invasion and metastasis of mouse forestomach carcinoma gastric cancer cell, which is related to The NF-κB signal pathway. |
| File Format | HTM / HTML |
| ISSN | 19957645 |
| e-ISSN | 23524146 |
| Journal | Asian Pacific Journal of Tropical Medicine |
| Issue Number | 9 |
| Volume Number | 8 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-01 |
| Publisher Place | China |
| Access Restriction | Open |
| Subject Keyword | Discipline Tropical Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
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