NDLI: Development of a 25-plex SNP assay for traceability in cattle.

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Development of a 25-plex SNP assay for traceability in cattle.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Karniol, B. Shirak, A. Baruch, E. Singrün, C. Tal, A. Cahana, A. Kam, M. Skalski, Y. Brem, G. Weller, J. I. Ron, M. Seroussi, E.
Description	Country affiliation: Israel Author Affiliation: Karniol B ( Agricultural Research Organization, Institute of Animal Science, Bet-Dagan, Israel.)
Abstract	Single nucleotide polymorphisms (SNPs) are amenable to automation and therefore have become the marker of choice for DNA profiling. SNaPshot, a primer extension-based method, was used to multiplex 25 SNPs that have been previously validated as useful for identity control. Detection of extended products was based on four different fluorochromes and extension primers with oligonucleotide tails of differing lengths, thus controlling the concise length of the entire chromatogram to 81 bases. Allele frequencies for Holstein, Simmental, Limousin, Angus, Charolais and Tux Cattle were estimated and significant positive Pearson-correlation coefficients were obtained among the analysed breeds. The probability that two randomly unrelated individuals would share identical genotypes for all 25 loci varied from 10(-8) to 10(-10) for these breeds. For parentage control, the exclusion power was found to be 99.9% when the genotypes of both putative parents are known. A traceability test of duplicated samples indicated a high genotyping precision of >0.998. This was further corroborated by analysis of 60 cases of parent-sib pairs and trio families. The 25-plex SNaPshot assay is adapted for low- and high-throughput capacity and thus presents an alternative for DNA-based traceability in the major commercial cattle breeds.
File Format	HTM / HTML
ISSN	02689146
Issue Number	3
Volume Number	40
e-ISSN	13652052
Journal	Animal Genetics
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2009-06-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Genetics Discipline Zoology Cattle Genetics Polymorphism, Single Nucleotide Animals Dna Chemistry Female Gene Frequency Genetic Variation Polymerase Chain Reaction Veterinary Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Animal Science and Zoology

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