NDLI: Genetic characterization, at the mitochondrial and nuclear DNA levels, of five Canary Island dog breeds.

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Genetic characterization, at the mitochondrial and nuclear DNA levels, of five Canary Island dog breeds.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Suárez, N. M. Betancor, E. Fregel, R. Pestano, J.
Spatial Coverage	Spain
Description	Country affiliation: Spain Author Affiliation: Suárez NM ( Department of Genetics, Faculty of Medicine, Universidad de Las Palmas de Gran Canaria, 35080, Las Palmas de Gran Canaria, Spain. nmartel@dbbf.ulpgc.es)
Abstract	Many studies presenting genetic analysis of dog breeds have been conducted without the inclusion of island dog breeds, although isolation can be one of the main factors in their origin. Here we report the genetic analysis at the nuclear and mitochondrial DNA levels of five Canary Island dog breeds (Canarian Warren Hound, Canary Island Mastiff, Garafiano Shepherd, La Palma Rat-Hunter and El Hierro Wolfhound) to fill this gap and, at the same time, genetically characterize these breeds. We identified 168 alleles in autosomal microsatellites and 16 mitochondrial haplotypes. Observed and expected heterozygosities ranged from 0.556 to 0.783 and from 0.737 to 0.943 respectively. Furthermore, three haplotypes were newly described and exclusive to a particular breed (A17+ in the Canary Island Mastiff; A33+ in the Canarian Warren Hound; Bi in the La Palma Rat-Hunter). The outcome of our analyses also revealed different breed histories consistent with historical documents and hypothetical origin designations. Although mtDNA haplotypes showed poor breed discriminating power, autosomal markers allowed a clear clustering of each single population. We expect that our results, together with further analyses, will help to make the population histories of island dog breeds clearer.
File Format	HTM / HTML
ISSN	02689146
Issue Number	4
Volume Number	44
e-ISSN	13652052
Journal	Animal Genetics
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2013-08-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Genetics Discipline Zoology Dna Genetics Dogs Genetic Variation Microsatellite Repeats Alleles Animals Base Sequence Breeding Cluster Analysis Chemistry Dna Primers Dna, Mitochondrial Classification Genetic Markers Genetics, Population Haplotypes Molecular Sequence Data Phylogeny Sequence Alignment Veterinary Sequence Analysis, Dna Spain Species Specificity Journal Article
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Animal Science and Zoology

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