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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gómez-Cortés, Pilar Tyburczy, Cynthia Brenna, J. Thomas Juárez, Manuela de la Fuente, Miguel Angel |
| Description | Country affiliation: Spain Author Affiliation: Gómez-Cortés P ( Instituto del Frío (Consejo Superior de Investigaciones Científicas), José Antonio Novais 10, 28040 Madrid, Spain.) |
| Abstract | Rumen biohydrogenation of dietary alpha-linolenic acid gives rise in ruminants to accumulation of fatty acid intermediates, some of which may be transferred into milk. Rumelenic acid [cis-9 trans-11 cis-15 C18:3 (RLnA)] has recently been characterized, but other C18:3 minor isomers are still unknown. The objective of this work was to identify a new isomer of octatridecenoic acid present in milk fat from ewes fed different sources of alpha-linolenic acid. Structural characterization of this fatty acid was achieved by GC-MS. Analysis of dimethyloxazoline and picolinyl ester derivatives allowed for location of the double bond positions. Covalent adduct chemical ionization tandem mass spectrometry confirmed the positional structure 9-11-15, identical to RLnA, and helped to establish double bond geometry (cis-trans-trans). This new C18:3 isomer could be formed by isomerization of cis-15 bond of RLnA and subsequently converted by hydrogenation to trans-11 trans-15 C18:2, an octadecadienoic acid also detected in this study. |
| File Format | HTM / HTML |
| ISSN | 00222275 |
| e-ISSN | 15397262 |
| DOI | 10.1194/jlr.M800662-JLR200 |
| Journal | The Journal of Lipid Research |
| Issue Number | 12 |
| Volume Number | 50 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2009-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Biochemistry Fatty Acids Chemistry Animals Chromatography, Gas Diet Dietary Supplements Stereoisomerism Tandem Mass Spectrometry Alpha-linolenic Acid Administration & Dosage Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Endocrinology |
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