NDLI: Changes in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N-acyl chain.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pinto, Sandra N. Laviad, Elad L. Stiban, Johnny Kelly, Samuel L. Merrill, Alfred H. Prieto, Manuel Futerman, Anthony H. Silva, Liana C.
Description	Country affiliation: Portugal Author Affiliation: Pinto SN ( Centro de Química-Física Molecular and Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal.)
Abstract	Ceramide (Cer) is involved in the regulation of several cellular processes by mechanisms that depend on Cer-induced changes on membrane biophysical properties. Accumulating evidence shows that Cers with different N-acyl chain composition differentially impact cell physiology, which may in part be due to specific alterations in membrane biophysical properties. We now address how the sphingolipid (SL) N-acyl chain affects membrane properties in cultured human embryonic kidney cells by overexpressing different Cer synthases (CerSs). Our results show an increase in the order of cellular membranes in CerS2-transfected cells caused by the enrichment in very long acyl chain SLs. Formation of Cer upon treatment of cells with bacterial sphingomyelinase promoted sequential changes in the properties of the membranes: after an initial increase in the order of the fluid plasma membrane, reorganization into domains with gel-like properties whose characteristics are dependent on the acyl chain structure of the Cer was observed. Moreover, the extent of alterations of membrane properties correlates with the amount of Cer formed. These data reinforce the significance of Cer-induced changes on membrane biophysical properties as a likely molecular mechanism by which different acyl chain Cers exert their specific biological actions.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.M042002
Journal	The Journal of Lipid Research
Issue Number	1
Volume Number	55
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2014-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Cell Membrane Metabolism Ceramides Biosynthesis Sphingomyelin Phosphodiesterase Physiology Fluorescence Polarization Hek293 Cells Membrane Proteins Sphingolipids Sphingosine N-acyltransferase Tumor Suppressor Proteins Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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