| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Duarte, Joao A. G. Carvalho, Filipa Pearson, Mackenzie Horton, Jay D. Browning, Jeffrey D. Jones, John G. Burgess, Shawn C. |
| Description |
Country affiliation: Portugal Author Affiliation: Duarte JA ( Advanced Imaging Research Center-Division of Metabolic Mechanisms of Disease, The University of Texas Southwestern Medical Center, Dallas, TX Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal.); Carvalho F ( Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal.); Pearson M ( Advanced Imaging Research Center-Division of Metabolic Mechanisms of Disease, The University of Texas Southwestern Medical Center, Dallas, TX.); Horton JD ( Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX.); Browning JD ( Advanced Imaging Research Center-Division of Metabolic Mechanisms of Disease, The University of Texas Southwestern Medical Center, Dallas, TX Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX.); Jones JG ( Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal.); Burgess SC ( Advanced Imaging Research Center-Division of Metabolic Mechanisms of Disease, The University of Texas Southwestern Medical Center, Dallas, TX Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX.) |
| Abstract |
Intracellular lipids and their synthesis contribute to the mechanisms and complications of obesity-associated diseases. We describe an NMR approach that provides an abbreviated lipidomic analysis with concurrent lipid biosynthetic fluxes. Following deuterated water administration, positional isotopomer analysis by deuterium NMR of specific lipid species was used to examine flux through de novo lipogenesis (DNL), FA elongation, desaturation, and TG-glycerol synthesis. The NMR method obviated certain assumptions regarding sites of enrichment and exchangeable hydrogens required by mass isotope methods. The approach was responsive to genetic and pharmacological gain or loss of function of DNL, elongation, desaturation, and glyceride synthesis. BDF1 mice consuming a high-fat diet (HFD) or matched low-fat diet for 35 weeks were examined across feeding periods to determine how flux through these pathways contributes to diet induced fatty liver and obesity. HFD mice had increased rates of FA elongation and glyceride synthesis. However DNL was markedly suppressed despite insulin resistance and obesity. We conclude that most hepatic TGs in the liver of HFD mice were formed from the reesterification of existing or ingested lipids, not DNL. |
| File Format |
HTM / HTML |
| ISSN |
00222275 |
| e-ISSN |
15397262 |
| DOI |
10.1194/jlr.M052308 |
| Journal |
The Journal of Lipid Research |
| Issue Number |
12 |
| Volume Number |
55 |
| Language |
English |
| Publisher |
American Society for Biochemistry and Molecular Biology |
| Publisher Date |
2014-12-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Biochemistry Diet, High-fat Adverse Effects Down-regulation Fatty Acid Desaturases Metabolism Lipogenesis Liver Non-alcoholic Fatty Liver Disease Obesity Adipose Tissue, White Enzymology Animals Crosses, Genetic Deuterium Esterification Genetics Fatty Acid Synthases Insulin Resistance Magnetic Resonance Spectroscopy Mice, Inbred C57bl Mice, Inbred Dba Mice, Transgenic Etiology Sterol Regulatory Element Binding Protein 1 Triglycerides Up-regulation Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Cell Biology Biochemistry Endocrinology |
