NDLI: Sculpting of DNA at abasic sites by DNA glycosylase homolog mag2.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dalhus, Bjørn Nilsen, Line Korvald, Hanne Huffman, Joy Forstrøm, Rune Johansen McMurray, Cynthia T. Alseth, Ingrun Tainer, John A. Bjørås, Magnar
Description	Country affiliation: Norway Author Affiliation: Dalhus B ( Department of Microbiology, Centre of Molecular Biology and Neuroscience, Oslo University Hospital, Rikshospitalet, PO Box 4950, Nydalen, N-0424, Oslo, Norway.)
Abstract	Modifications and loss of bases are frequent types of DNA lesions, often handled by the base excision repair (BER) pathway. BER is initiated by DNA glycosylases, generating abasic (AP) sites that are subsequently cleaved by AP endonucleases, which further pass on nicked DNA to downstream DNA polymerases and ligases. The coordinated handover of cytotoxic intermediates between different BER enzymes is most likely facilitated by the DNA conformation. Here, we present the atomic structure of Schizosaccharomyces pombe Mag2 in complex with DNA to reveal an unexpected structural basis for nonenzymatic AP site recognition with an unflipped AP site. Two surface-exposed loops intercalate and widen the DNA minor groove to generate a DNA conformation previously only found in the mismatch repair MutS-DNA complex. Consequently, the molecular role of Mag2 appears to be AP site recognition and protection, while possibly facilitating damage signaling by structurally sculpting the DNA substrate.
File Format	HTM / HTML
ISSN	09692126
e-ISSN	18784186
DOI	10.1016/j.str.2012.11.004
Journal	Structure
Issue Number	1
Volume Number	21
Language	English
Publisher	Elsevier
Publisher Date	2013-01-08
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Discipline Biotechnology Apurinic Acid Chemistry Dna Glycosylases Schizosaccharomyces Pombe Proteins Schizosaccharomyces Enzymology Amino Acid Sequence Base Pairing Binding Sites Crystallography, X-ray Genetics Metabolism Dna Repair Dna, Fungal Epistasis, Genetic Gene Knockout Techniques Models, Molecular Molecular Sequence Data Muts Dna Mismatch-binding Protein Nucleic Acid Conformation Protein Binding Protein Structure, Secondary Protein Structure, Tertiary Sequence Homology, Amino Acid Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Structural Biology Molecular Biology

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Unprecedented Sculpting of DNA at Abasic Sites by DNA Glycosylase Homolog Mag2

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