NDLI: Dramatic improvement of crystals of large RNAs by cation replacement and dehydration

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Dramatic improvement of crystals of large RNAs by cation replacement and dehydration

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Dramatic improvement of crystals of large RNAs by cation replacement and dehydration

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Jinwei Ferré-D'Amaré, Adrian R.
Description	Country affiliation: United States Author Affiliation: Zhang J ( National Heart, Lung and Blood Institute, 50 South Drive, MSC 8012, Bethesda, MD 20892-8012, USA.); Ferré-D'Amaré AR ( National Heart, Lung and Blood Institute, 50 South Drive, MSC 8012, Bethesda, MD 20892-8012, USA. Electronic address: adrian.ferre@nih.gov.)
Abstract	Compared to globular proteins, RNAs with complex 3D folds are characterized by poorly differentiated molecular surfaces dominated by backbone phosphates, sparse tertiary contacts stabilizing global architecture, and conformational flexibility. The resulting generally poor order of crystals of large RNAs and their complexes frequently hampers crystallographic structure determination. We describe and rationalize a postcrystallization treatment strategy that exploits the importance of solvation and counterions for RNA folding. Replacement of Li(+) and Mg(2+) needed for growth of crystals of a tRNA-riboswitch-protein complex with Sr(2+), coupled with dehydration, dramatically improved the resolution limit (8.5-3.2 Å) and data quality, enabling structure determination. The soft Sr(2+) ion forms numerous stabilizing intermolecular contacts. Comparison of pre- and posttreatment structures reveals how RNA assemblies redistribute as quasi-rigid bodies to yield improved crystal packing. Cation exchange complements previously reported postcrystallization dehydration of protein crystals and represents a potentially general strategy for improving crystals of large RNAs.
File Format	HTM / HTML
ISSN	09692126
e-ISSN	18784186
DOI	10.1016/j.str.2014.07.011
Journal	Structure
Issue Number	9
Volume Number	22
Language	English
Publisher	Elsevier
Publisher Date	2014-09-02
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Discipline Biotechnology Rna, Transfer Chemistry Crystallography, X-ray Desiccation Lithium Compounds Magnesium Models, Molecular Nucleic Acid Conformation Riboswitch Strontium Sulfates Research Support, N.i.h., Extramural Research Support, N.i.h., Intramural Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Structural Biology Molecular Biology

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Dramatic improvement of crystals of large RNAs by cation replacement and dehydration

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Dramatic improvement of crystals of large RNAs by cation replacement and dehydration

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