NDLI: Distinct conformational spectrum of homologous multidrug ABC transporters.

Please wait, while we are loading the content...

Year: 2017 Volume: 25

Year: 2016 Volume: 24

Year: 2015 Volume: 23

Year: 2015 Volume: 23 Issue: 12

Year: 2015 Volume: 23 Issue: 11

Year: 2015 Volume: 23 Issue: 10

Year: 2015 Volume: 23 Issue: 9

Year: 2015 Volume: 23 Issue: 8

Year: 2015 Volume: 23 Issue: 7

Year: 2015 Volume: 23 Issue: 6

Year: 2015 Volume: 23 Issue: 5

Year: 2015 Volume: 23 Issue: 4

Year: 2015 Volume: 23 Issue: 3

Distinct conformational spectrum of homologous multidrug ABC transporters.

Structural basis for dodecameric assembly states and conformational plasticity of the full-length AAA+ ATPases Rvb1 · Rvb2.

Insights into Cullin-RING E3 ubiquitin ligase recruitment: structure of the VHL-EloBC-Cul2 complex.

Structure of the vacuolar H+-ATPase rotary motor reveals new mechanistic insights.

Structure of human NAPE-PLD: regulation of fatty-acid ethanolamide biosynthesis by bile acids

Allosteric effects of the oncogenic RasQ61L mutant on Raf-RBD.

Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism.

A bipartite interaction between Hsp70 and CHIP regulates ubiquitination of chaperoned client proteins.

Lipopolysaccharide is inserted into the outer membrane through an intramembrane hole, a lumen gate, and the lateral opening of LptD.

A conserved activation cluster is required for allosteric communication in HtrA-family proteases.

Nothing to sneeze at: a dynamic and integrative computational model of an influenza A virion.

DNA-damage-inducible 1 protein (Ddi1) contains an uncharacteristic ubiquitin-like domain that binds ubiquitin.

The membrane- and soluble-protein helix-helix interactome: similar geometry via different interactions.

Structural insights into Ca2+-calmodulin regulation of Plectin 1a-integrin ß4 interaction in hemidesmosomes.

VHL: Cullin-g the hypoxic response.

Helix-helix interactions: is the medium the message?

Simulations move toward a cure for viral diseases.

Year: 2015 Volume: 23 Issue: 2

Year: 2015 Volume: 23 Issue: 1

Year: 2014 Volume: 22

Year: 2013 Volume: 21

Year: 2012 Volume: 20

Year: 2011 Volume: 19

Year: 2010 Volume: 18

Year: 2009 Volume: 17

Distinct conformational spectrum of homologous multidrug ABC transporters.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Moeller, Arne Lee, Sung Chang Tao, Houchao Speir, Jeffrey A. Chang, Geoffrey Urbatsch, Ina L. Potter, Clinton S. Carragher, Bridget Zhang, Qinghai
Description	Country affiliation: United States Author Affiliation: Moeller A ( Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA); Lee SC ( Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.); Tao H ( Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.); Speir JA ( Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA); Chang G ( Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.); Urbatsch IL ( Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430, USA.); Potter CS ( Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA); Carragher B ( Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA); Zhang Q ( Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. Electronic address: qinghai@scripps.edu.)
Abstract	ATP-binding cassette (ABC) exporters are ubiquitously found in all kingdoms of life and their members play significant roles in mediating drug pharmacokinetics and multidrug resistance in the clinic. Significant questions and controversies remain regarding the relevance of their conformations observed in X-ray structures, their structural dynamics, and mechanism of transport. Here, we used single particle electron microscopy (EM) to delineate the entire conformational spectrum of two homologous ABC exporters (bacterial MsbA and mammalian P-glycoprotein) and the influence of nucleotide and substrate binding. Newly developed amphiphiles in complex with lipids that support high protein stability and activity enabled EM visualization of individual complexes in a membrane-mimicking environment. The data provide a comprehensive view of the conformational flexibility of these ABC exporters under various states and demonstrate not only similarities but striking differences between their mechanistic and energetic regulation of conformational changes.
File Format	HTM / HTML
ISSN	09692126
e-ISSN	18784186
DOI	10.1016/j.str.2014.12.013
Journal	Structure
Issue Number	3
Volume Number	23
Language	English
Publisher	Elsevier
Publisher Date	2015-03-03
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Discipline Biotechnology Atp-binding Cassette Transporters Ultrastructure Bacterial Proteins P-glycoprotein Chemistry Animals Membrane Lipids Mice Microscopy, Electron Models, Molecular Nucleotides Protein Binding Protein Conformation Protein Stability Structural Homology, Protein Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Structural Biology Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Distinct Conformational Spectrum of Homologous Multidrug ABC Transporters

Insights into how nucleotide-binding domains power ABC transport.

ABC multidrug transporters in schistosomes and other parasitic flatworms.

Molecular basis of multidrug transport by ABC transporters

Structural consequences of ATP hydrolysis on the ABC transporter NBD dimer: molecular dynamics studies of HlyB.

Mechanism of ABC transporters: a molecular dynamics simulation of a well characterized nucleotide-binding subunit.

Efficient purification and reconstitution of ATP binding cassette transporter B6 (ABCB6) for functional and structural studies.

Ovarian hormones modulate multidrug resistance transporters in the ovary

Multidrug resistance ATP-binding cassette membrane transporters as targets for improving oropharyngeal candidiasis treatment

Distinct conformational spectrum of homologous multidrug ABC transporters.

Similar Documents

Distinct Conformational Spectrum of Homologous Multidrug ABC Transporters

Insights into how nucleotide-binding domains power ABC transport.

ABC multidrug transporters in schistosomes and other parasitic flatworms.

Molecular basis of multidrug transport by ABC transporters

Structural consequences of ATP hydrolysis on the ABC transporter NBD dimer: molecular dynamics studies of HlyB.

Mechanism of ABC transporters: a molecular dynamics simulation of a well characterized nucleotide-binding subunit.

Efficient purification and reconstitution of ATP binding cassette transporter B6 (ABCB6) for functional and structural studies.

Ovarian hormones modulate multidrug resistance transporters in the ovary

Multidrug resistance ATP-binding cassette membrane transporters as targets for improving oropharyngeal candidiasis treatment

Distinct conformational spectrum of homologous multidrug ABC transporters.