NDLI: Dynamics of constitutive heterochromatin: two contrasted kinetics of genome restructuring in early cloned bovine embryos.

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Dynamics of constitutive heterochromatin: two contrasted kinetics of genome restructuring in early cloned bovine embryos.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pichugin, Andrey Le Bourhis, Daniel Adenot, Pierre Lehmann, Gaëtan Audouard, Christophe Renard, Jean-Paul Vignon, Xavier Beaujean, Nathalie
Description	Country affiliation: France Author Affiliation: Pichugin A ( INRA, UMR 1198 Biologie du développement et reproduction, F-78350 Jouy en Josas, France.)
Abstract	Efficient reprograming of the donor cell genome in nuclear transfer (NT) embryos is linked to the ability of the embryos to sustain full-term development. As the nuclear architecture has recently emerged as a key factor in the regulation of gene expression, we questioned whether early bovine embryos obtained from transfer of cultured fibroblasts into enucleated oocytes would adopt an embryo-like nuclear organization. We studied the dynamics of constitutive heterochromatin in the stages prior to embryonic genome activation by distribution analysis of heterochromatin protein CBX1 (HP1), centromeric proteins CENPA and CENPB, and histone H3 three-methylated at lysine 9. Then we applied descriptive, quantitative, and co-localization analyses. A dramatic reorganization of heterochromatic blocks of somatic donor cells was first observed in the late one-cell stage NT embryos. Then at two- and four-cell stages, we found two types of NT embryos: one displaying noncondensed heterochromatin patches similar to IVF embryos, whereas the second type displayed condensed heterochromatin blocks, normally observed in IVF embryos only after the eight-cell stage. These analyses discriminate for the first time two contrasted types of nuclear organization in NT embryos, which may correspond to different functional states of the nuclei. The relationship with the somatic nucleus reprograming efficiency is discussed.
File Format	HTM / HTML
ISSN	14701626
e-ISSN	17417899
Journal	Reproduction
Issue Number	1
Volume Number	139
Language	English
Publisher	BioScientifica
Publisher Date	2010-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Reproductive Medicine Chromatin Assembly And Disassembly Embryo, Mammalian Metabolism Embryonic Development Heterochromatin Animals Autoantigens Cell Nucleus Ultrastructure Cells, Cultured Cellular Reprogramming Centromere Protein B Chromosomal Proteins, Non-histone Cloning, Organism Cytoplasmic Granules Fertilization In Vitro Fibroblasts Classification Histones Kinetics Microscopy, Confocal Nuclear Transfer Techniques Oocytes Comparative Study Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Embryology Endocrinology Reproductive Medicine Obstetrics and Gynecology

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