NDLI: Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa.

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Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jimenez, Tamara Sánchez, Gladis Wertheimer, Eva Blanco, Gustavo
Description	Country affiliation: United States Author Affiliation: Jimenez T ( Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA.)
Abstract	While the function of the ubiquitous Na,K-ATPase alpha1 subunit has been well documented, the role of the sperm-specific alpha4 isoform of this ion transporter is less known. We have explored the importance of alpha4 in rat sperm physiology by taking advantage of the high sensitivity of this isoform for the inhibitor ouabain. Using concentrations that selectively block alpha4 activity, we found ouabain to reduce not only sperm total motility, but also multiple parameters of sperm movement, including progressive motility, straight line, curvilinear, and average path velocities, lateral head displacement, beat cross frequency, and linearity. According to a direct role of alpha4 in Na(+) transport, ouabain inhibition of alpha4 increased [Na(+)](i) in the male gametes. In addition, interference of alpha4 activity with ouabain produced cell membrane depolarization, diminished pH, and increased [Ca(2)(+)](i) in spermatozoa. Inhibition of alpha4 was sufficient to cause all these effects and additional blockage of alpha1, the other Na,K-ATPase alpha isoform expressed in sperm, and higher doses of ouabain did not result in further changes in the cell parameters studied. These results show that alpha4 is the Na,K-ATPase isoform primarily involved in controlling the transmembrane Na(+) gradient in sperm, and that alpha4 activity is necessary for maintaining membrane potential, [Ca(2)(+)](i), and [H(+)](i) in the cells. The high dependence of sperm motility on membrane excitability, [Ca(2)(+)](i), and acid-base balance suggests that their regulation is the mechanism by which alpha4 maintains motility of the male gametes.
File Format	HTM / HTML
ISSN	14701626
e-ISSN	17417899
Journal	Reproduction
Issue Number	5
Volume Number	139
Language	English
Publisher	BioScientifica
Publisher Date	2010-05-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Reproductive Medicine Calcium Signaling Membrane Potentials Sodium-potassium-exchanging Atpase Physiology Sperm Motility Spermatozoa Enzymology Animals Drug Effects Dose-response Relationship, Drug Enzyme Inhibitors Hydrogen-ion Concentration Isoenzymes Antagonists & Inhibitors Ouabain Semen Analysis Sodium Metabolism Time Factors Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Embryology Endocrinology Reproductive Medicine Obstetrics and Gynecology

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