NDLI: The photoinduced transformation of fluorescent DNA base analogue tC triggers DNA melting

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Effects of dynamic disorder on exciton delocalization and photoinduced charge separation in DNA.

Wavelength-dependent ultraviolet induction of cyclobutane pyrimidine dimers in the human cornea.

Solar-UV-signature mutation prefers TCG to CCG: extrapolative consideration from UVA1-induced mutation spectra in mouse skin.

DNA damage as a biological sensor for environmental sunlight.

Role of intersystem crossing in the fluorescence quenching of 2-aminopurine 2'-deoxyriboside in solution.

The solvent effect and identification of a weakly emissive state in nonradiative dynamics of guanine nucleosides and nucleotides--a combined femtosecond broadband time-resolved fluorescence and transient absorption study.

Electronic coupling between photo-excited stacked bases in DNA and RNA strands with emphasis on the bright states initially populated.

Sensitized photochemistry of di(4-tetrazolouracil) dinucleoside monophosphate as a route to dicytosine cyclobutane photoproduct precursors.

The variety of UV-induced pyrimidine dimeric photoproducts in DNA as shown by chromatographic quantification methods.

A joint experimental/theoretical study of the ultrafast excited state deactivation of deoxyadenosine and 9-methyladenine in water and acetonitrile.

Photochemistry and photophysics of the amino and imino tautomers of 1-methylcytosine: tautomerisation as a side product of the radiationless decay.

The photoinduced transformation of fluorescent DNA base analogue tC triggers DNA melting

Strong impact of the solvent on the photokinetics of a 2(1H)-pyrimidinone.

Electronic excited states of guanine-cytosine hairpins and duplexes studied by fluorescence spectroscopy.

Electronic excitation and structural relaxation of the adenine dinucleotide in gas phase and solution.

Photophysical properties of 5-substituted 2-thiopyrimidines.

Ultrafast electronic deactivation dynamics of the inosine dimer--a model case for H-bonded purine bases.

Accumulation of the cyclobutane thymine dimer in defined sequences of free and nucleosomal DNA.

The relative roles of DNA damage induced by UVA irradiation in human cells.

The effect of dimerization on the excited state behavior of methylated xanthine derivatives: a computational study.

UV-induced formation of the thymine-thymine pyrimidine (6-4) pyrimidone photoproduct--a DFT study of the oxetane intermediate ring opening.

Role of intercalation and redox potential in DNA photosensitization by ruthenium(II) polypyridyl complexes: assessment using DNA repair protein tests.

The interplay between neutral exciton and charge transfer states in single-strand polyadenine: a quantum dynamical investigation.

Interaction of UV radiation with DNA.

Cytosine containing dipyrimidine sites can be hotspots of cyclobutane pyrimidine dimer formation after UVB exposure.

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The photoinduced transformation of fluorescent DNA base analogue tC triggers DNA melting

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Preus, Søren Jønck, Søren Pittelkow, Michael Dierckx, Anke Karpkird, Thitinun Albinsson, Bo Wilhelmsson, L. Marcus
Description	Country affiliation: Denmark Author Affiliation: Preus S ( Department of Chemistry, University of Copenhagen, Copenhagen, DK-2100, Denmark.)
Abstract	While fluorescent analogues of the canonical nucleobases have proven to be highly valuable in a large number of applications, up until today, fluorescent DNA base analogues remain virtually inapplicable for single-molecule fluorescence experiments which require extremely bright and photostable dyes . Insight into the photodegradation processes of these fluorophores is thus a key step in the continuous development towards dyes with improved performances. Here, we show that the commercially available fluorescent nucleobase analogue tC under intense long-term illumination and in the presence of $O_{2}$ is degraded to form a single photoreaction product which we suggest to be the sulfoxide form of tC. The photoproduct is characterized by a blue-shifted absorption and a less intense fluorescence compared to that of tC. Interestingly, when tC is positioned inside double-stranded DNA this photodriven conversion of tC to its photoproduct greatly reduces the duplex stability of the overall double helix in which the probe is positioned. Since tC can be excited selectively at 400 nm, well outside the absorption band of the natural DNA bases, this observation points towards the application of tC as a general light-triggered switch of DNA duplex stability.
File Format	HTM / HTML
ISSN	1474905X
Issue Number	8
Volume Number	12
e-ISSN	14749092
Journal	Photochemical & Photobiological Sciences
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2013-08-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Chemistry Discipline Biology Dna Chemistry Fluorescent Dyes Phenothiazines Cytosine Analogs & Derivatives Light Models, Molecular Nucleic Acid Denaturation Photolysis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

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