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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pottosin, Igor I. Bonales-Alatorre, Edgar Valencia-Cruz, Georgina Mendoza-Magaña, Maria Luisa Dobrovinskaya, Oxana R. |
| Description | Country affiliation: Mexico Author Affiliation: Pottosin II ( Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Av. 25 de Julio 965, Villa San Sebastian, 28045, Colima, Mexico.) |
| Abstract | In this study, we present patch-clamp characterization of the background potassium current in human lymphoma (Jurkat cells), generated by voltage-independent 16 pS channels with a high (∼100-fold) $K^{+}/Na^{+}$ selectivity. Depending on the background $K^{+}$ channels density, from few per cell up to ∼1 open channel per $μm^{2},$ resting membrane potential was in the range of −40 to −83 mV, approaching $E_{K} = −88 mV.$ The background $K^{+}$ channels were insensitive to margotoxin (3 nM), apamine (3 nM), and clotrimazole (1 μM), high-affinity blockers of the lymphocyte Kv1.3, SKCa2, and IKCa1 channels. The current depended weakly on external pH. Arachidonic acid (20 μM) and $Hg^{2+}$ (0.3–10 μM) suppressed background $K^{+}$ current in Jurkat cells by 75–90%. Background $K^{+}$ current was weakly sensitive to $TEA^{+}$ $(IC_{50} = 14 mM),$ and was efficiently suppressed by externally applied bupivacaine $(IC_{50} = 5 μM),$ quinine $(IC_{50} = 16 μM),$ and $Ba^{2+}$ (2 mM). Our data, in particular strong inhibition by mercuric ions, suggest that background $K^{+}$ currents expressed in Jurkat cells are mediated by TWIK-related spinal cord $K^{+}$ (TRESK) channels belonging to the double-pore domain $K^{+}$ channel family. The presence of human TRESK in the membrane protein fraction was confirmed by Western blot analysis. |
| File Format | HTM / HTML |
| ISSN | 00316768 |
| Issue Number | 6 |
| Volume Number | 456 |
| e-ISSN | 14322013 |
| Journal | Pflügers Archiv - European Journal of Physiology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2008-09-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Physiology Potassium Channels Physiology Blotting, Western Electrophysiology Humans Jurkat Cells Kv1.3 Potassium Channel Chemistry Drug Effects Metabolism Membrane Proteins Isolation & Purification Patch-clamp Techniques Potassium Channel Blockers Pharmacology Potassium Channels, Calcium-activated Spinal Cord Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Physiology (medical) Clinical Biochemistry |
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