NDLI: Altered myoplasmic $Ca^{2+}$ handling in rat fast-twitch skeletal muscle fibres during disuse atrophy

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Altered myoplasmic $Ca^{2+}$ handling in rat fast-twitch skeletal muscle fibres during disuse atrophy

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Weiss, Norbert Andrianjafiniony, Tina Dupré-Aucouturier, Sylvie Pouvreau, Sandrine Desplanches, Dominique Jacquemond, Vincent
Description	Country affiliation: France Author Affiliation: Weiss N ( Physiologie Intégrative Cellulaire et Moléculaire, Université Lyon 1, UMR CNRS 5123, Bât. Raphaël Dubois, 43 boulevard du 11 novembre 1918, 69622, Villeurbanne, France.)
Abstract	Calcium-dependent signalling pathways are believed to play an important role in skeletal muscle atrophy, but whether intracellular $Ca^{2+}$ homeostasis is affected in that situation remains obscure. We show here that there is a 20% atrophy of the fast-type flexor digitorum brevis (FDB) muscle in rats hind limb unloaded (HU) for 2 weeks, with no change in fibre type distribution. In voltage-clamp experiments, the amplitude of the slow $Ca^{2+}$ current was found similar in fibres from control and HU animals. In fibres loaded with the $Ca^{2+}$ dye indo-1, the value for the rate of $[Ca^{2+}]$ decay after the end of 5–100-ms-long voltage-clamp depolarisations from −80 to +10 mV was found to be 30–50% lower in fibres from HU animals. This effect was consistent with a reduced contribution of both saturable and non-saturable components of myoplasmic $Ca^{2+}$ removal. However, there was no change in the relative amount of parvalbumin, and type 1 sarco-endoplasmic reticulum $Ca^{2+}-ATPase$ was increased by a factor of three in the atrophied muscles. Confocal imaging of mitochondrial membrane potential showed that atrophied FDB fibres had significantly depolarized mitochondria as compared to control fibres. Depolarization of mitochondria in control fibres with carbonyl cyanide-p-trifluoromethoxyphenylhydrazone induced a slowing of the decay of $[Ca^{2+}]$ transients accompanied by an increase in resting $[Ca^{2+}]$ and a reduction of the peak amplitude of the transients. Overall results provide the first functional evidence for severely altered intracellular $Ca^{2+}$ removal capabilities in atrophied fast-type muscle fibres and highlight the possible contribution of reduced mitochondrial polarisation.
File Format	HTM / HTML
ISSN	00316768
Issue Number	4
Volume Number	459
e-ISSN	14322013
Journal	Pflügers Archiv - European Journal of Physiology
Language	English
Publisher	Springer
Publisher Date	2010-03-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Physiology Calcium Metabolism Muscle Fibers, Fast-twitch Muscular Disorders, Atrophic Animals Carbonyl Cyanide P-trifluoromethoxyphenylhydrazone Female Fluorescent Dyes Hindlimb Suspension Indoles Ionophores Membrane Potential, Mitochondrial Physiology Cytology Pathology Patch-clamp Techniques Rats Rats, Wistar Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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Altered myoplasmic Ca2+ handling in rat fast-twitch skeletal muscle fibres during disuse atrophy

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Altered myoplasmic $Ca^{2+}$ handling in rat fast-twitch skeletal muscle fibres during disuse atrophy

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Altered myoplasmic Ca2+ handling in rat fast-twitch skeletal muscle fibres during disuse atrophy

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Altered myoplasmic $Ca^{2+}$ handling in rat fast-twitch skeletal muscle fibres during disuse atrophy